TBP Polyclonal antibody

TBP Polyclonal Antibody for WB,IP,IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Rat and More (3)

Applications

WB,IP,IHC,ELISA

Conjugate

Unconjugated

Cat no : 22006-1-AP

Synonyms

GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID


Filter:
  • Western Blot (WB) analysis of COLO 320 cells using TBP Polyclonal antibody (22006-1-AP)

    WB analysis of COLO 320 using 22006-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 22006-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using TBP Polyclonal antibody (22006-1-AP)

    WB analysis of COLO 320 using 22006-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 22006-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using TBP Polyclonal antibody (22006-1-AP)

    WB analysis of COLO 320 using 22006-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 22006-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using TBP Polyclonal antibody (22006-1-AP)

    IP experiment of COLO 320 using 22006-1-AP

    IP result of anti-TBP (IP:22006-1-AP, 4ug; Detection:22006-1-AP 1:500) with COLO 320 cells lysate 2560 ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TBP Polyclonal antibody (22006-1-AP)

    IHC staining of mouse testis using 22006-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22006-1-AP (TBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TBP Polyclonal antibody (22006-1-AP)

    IHC staining of mouse testis using 22006-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22006-1-AP (TBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using TBP Polyclonal antibody (22006-1-AP)

    IHC staining of rat liver using 22006-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 22006-1-AP (TBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBP Polyclonal antibody (22006-1-AP)

    IHC staining of human breast cancer using 22006-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22006-1-AP (TBP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

"TBP Antibodies" Comparison

View side-by-side comparison of TBP antibodies from other vendors to find the one that best suits your research needs.

Tested Applications

Positive WB detected inCOLO 320 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inmouse testis tissue, human breast cancer tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22006-1-AP targets TBP in WB,IP,IHC,ELISA applications and shows reactivity with human, mouse, Rat samples.

Tested Reactivity human, mouse, Rat
Cited Reactivityhuman, mouse, rat, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBP fusion protein Ag12383
Full Name TATA box binding protein
Calculated Molecular Weight 338 aa, 38 kDa
Observed Molecular Weight mouse/rat 33-36 kDa and human 37-43kDa
GenBank Accession NumberBC110341
Gene Symbol TBP
Gene ID (NCBI) 6908
RRIDAB_10951514
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.

Protocols

Product Specific Protocols
WB protocol for TBP antibody 22006-1-APDownload protocol
IHC protocol for TBP antibody 22006-1-APDownload protocol
IP protocol for TBP antibody 22006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Hepatology

Steatotic hepatocytes release mature VLDL via methionine and tyrosine metabolism in a Keap1-Nrf2 dependent manner.

Authors - Akitoshi Sano
humanWB

Nat Commun

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Authors - Gongwei Wu
humanWB

Elife

KPNB1 mediates PER/CRY nuclear translocation and circadian clock function.

Authors - Yool Lee
humanWB

J Hazard Mater

ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes.

Authors - Xuan Lai
humanWB

Cell Death Dis

4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer

Authors - Yanan Wang
humanWB

EMBO Rep

LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1α-FOXO4-p21-dependent signaling axis.

Authors - Xuedan Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Mi (Verified Customer) (06-25-2023)

This antibody works very well in human white and brown adipocyte cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: human adipocytes