TBP Polyclonal antibody

TBP Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Rat and More (4)

Applications

WB, IHC, IF, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

76

Reviews (1)

Cat no : 22006-1-AP

Synonyms

GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis of COLO 320 using 22006-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22006-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 22006-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22006-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 22006-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22006-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 22006-1-AP

  IP result of anti-TBP (IP:22006-1-AP, 4ug; Detection:22006-1-AP 1:500) with COLO 320 cells lysate 2560 ug.

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  IHC staining of mouse testis using 22006-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22006-1-AP (TBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 22006-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22006-1-AP (TBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 22006-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 22006-1-AP (TBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22006-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22006-1-AP (TBP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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Tested Applications

• Positive WB detected in: COLO 320 cells
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: mouse testis tissue, human breast cancer tissue, rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 70 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Product Information

22006-1-AP targets TBP in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, Rat samples.

• Tested Reactivity: human, mouse, Rat
• Cited Reactivity: human, mouse, rat, pig, monkey, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBP fusion protein Ag12383
• Full Name: TATA box binding protein
• Calculated Molecular Weight: 338 aa, 38 kDa
• Observed Molecular Weight: mouse/rat 33-36 kDa and human 37-43kDa
• GenBank Accession Number: BC110341
• Gene Symbol: TBP
• Gene ID (NCBI): 6908
• RRID: AB_10951514
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.

Publications

Species	Application	Title
mouse	WB	Hepatology; Steatotic hepatocytes release mature VLDL via methionine and tyrosine metabolism in a Keap1-Nrf2 dependent manner.; Authors - Akitoshi Sano
mouse	WB,IP,IF	Redox Biol; Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke; Authors - Haiyun Guo
human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu
human	WB	Cancer Res; DNA of neutrophil extracellular traps binds TMCO6 to impair CD8+ T-cell immunity in hepatocellular carcinoma; Authors - Mengjia Song
human	WB	Elife; KPNB1 mediates PER/CRY nuclear translocation and circadian clock function.; Authors - Yool Lee
human	WB	J Hazard Mater; ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes.; Authors - Xuan Lai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mi (Verified Customer) (06-25-2023)

This antibody works very well in human white and brown adipocyte cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human adipocytes

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