Anticorps Polyclonal de lapin anti-ARF4

• Phare
• Validé par KD/KO

ARF4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

36

N° de cat : 11673-1-AP

Synonymes

ARF4, ADP ribosylation factor 4, ADP-ribosylation factor 4, ARF2

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Galerie de données de validation

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  WB analysis using 11673-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11673-1-AP (ARF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11673-1-AP

  WB result of ARF4 antibody (11673-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF4 transfected HeLa cells.

• 

  WB analysis using 11673-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11673-1-AP (ARF4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11673-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11673-1-AP (ARF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11673-1-AP

  IP result of anti-ARF4 (IP:11673-1-AP, 3ug; Detection:11673-1-AP 1:600) with mouse brain tissue lysate 5200ug.

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  IP experiment of HepG2 using 11673-1-AP

  IP result of anti-ARF4 (IP:11673-1-AP, 4ug; Detection:11673-1-AP 1:3000) with HepG2 cells lysate 1080 ug.

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  IHC staining of human breast cancer using 11673-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11673-1-AP (ARF4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11673-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11673-1-AP (ARF4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11673-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11673-1-AP (ARF4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of K-562 using 11673-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human ARF4 (11673-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules HeLa, cellules HepG2, cellules K-562, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HepG2, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules K-562
• Résultats positifs en cytométrie: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 32 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Informations sur le produit

11673-1-AP cible ARF4 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARF4 Protéine recombinante Ag2272
• Nom complet: ADP-ribosylation factor 4
• Masse moléculaire calculée: 180 aa, 21 kDa
• Poids moléculaire observé: 16 kDa, 21 kDa
• Numéro d’acquisition GenBank: BC003364
• Symbole du gène: ARF4
• Identification du gène (NCBI): 378
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARF4, also named as ARF2, belongs to the small GTPase superfamily and Arf family. ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. Their function are not fully described. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.; Authors - Reiling Jan H JH
human	WB	Sci Adv; p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.; Authors - Xiaochao Tan
mouse	WB	Nat Commun; Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.; Authors - Hyunho Kim
human	WB	Proc Natl Acad Sci U S A; Whole-genome RNAi screen highlights components of the endoplasmic reticulum/Golgi as a source of resistance to immunotoxin-mediated cytotoxicity.; Authors - Matteo Pasetto ; KD Validated
human	WB	Cell Rep; KDEL Receptors Assist Dengue Virus Exit from the Endoplasmic Reticulum.; Authors - Ming Yuan Li
human	WB	Oncogene; A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer.; Authors - Breege V Howley

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