iPLA2 Polyclonal antibody

iPLA2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 22030-1-AP

Synonyms

CaI PLA2, Group VI phospholipase A2, GVI, GVI PLA2, INAD1, iPLA2, iPLA2 beta, IPLA2 VIA, PARK14, PLA2, PLA2G6, PNPLA9

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Validation Data Gallery

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  WB analysis of mouse testis using 22030-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22030-1-AP (iPLA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 22030-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22030-1-AP (iPLA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 22030-1-AP

  IP result of anti-iPLA2 (IP:22030-1-AP, 4ug; Detection:22030-1-AP 1:800) with mouse testis tissue lysate 4000ug.

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  IHC staining of human small intestine using 22030-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22030-1-AP (iPLA2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 22030-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22030-1-AP (iPLA2 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 22030-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22030-1-AP (iPLA2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 22030-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22030-1-AP (iPLA2 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human small intestine tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

22030-1-AP targets iPLA2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: iPLA2 fusion protein Ag16896
• Full Name: phospholipase A2, group VI (cytosolic, calcium-independent)
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 80 kDa, 88 kDa
• GenBank Accession Number: BC036742
• Gene Symbol: PLA2G6
• Gene ID (NCBI): 8398
• RRID: AB_2878976
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLA2G6, also named as PLPLA9, is involved in the remodeling of membranes allowing arachidonic acid to be placed in the proper position in phospholipids for stimulated release. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. This protein has 4 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Cell Rep; Alpha synuclein determines ferroptosis sensitivity in dopaminergic neurons via modulation of ether-phospholipid membrane composition; Authors - Laura Mahoney-Sanchez
human,mouse	WB,IHC	J Agric Food Chem; Inhibitory Effects of Peroxidase from Foxtail Millet Bran on Colitis-Associated Colorectal Carcinogenesis by the Blockage of Glycerophospholipid Metabolism.; Authors - Shuhua Shan
rat	IF	Neurol Res; Calcium-independent phospholipase A2 inhibitor produces an analgesic effect in a rat model of neuropathic pain by reducing central sensitization in the dorsal horn.; Authors - Young Seob Gwak
human	IHC	Zhongguo Dang Dai Er Ke Za Zhi; [Clinical features of infantile neuroaxonal dystrophy and PLA2G6 gene testing].; Authors - Yao Lu
human	WB,IHC	Theranostics; Ferritin light chain promotes the reprogramming of glioma immune microenvironment and facilitates glioma progression; Authors - Hongjiang Li
human	IHC	Molecules; Identification of Diagnostic Metabolic Signatures in Thyroid Tumors Using Mass Spectrometry Imaging; Authors - Xinxin Mao