ARF5 Polyclonal antibody

• Featured Product
• KD/KO Validated

ARF5 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15281-1-AP

Synonyms

ADP-ribosylation factor 5, ADP ribosylation factor 5

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Validation Data Gallery

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  WB analysis of PC-12 using 15281-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15281-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15281-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15281-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15281-1-AP (ARF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 15281-1-AP

  IP result of anti-ARF5 (IP:15281-1-AP, 4ug; Detection:15281-1-AP 1:500) with MCF-7 cells lysate 2840 ug.

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  IHC staining of human breast cancer using 15281-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15281-1-AP (ARF5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15281-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15281-1-AP (ARF5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15281-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15281-1-AP (ARF5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

15281-1-AP targets ARF5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARF5 fusion protein Ag7400
• Full Name: ADP-ribosylation factor 5
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC003043
• Gene Symbol: ARF5
• Gene ID (NCBI): 381
• RRID: AB_2227435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

Publications

Species	Application	Title
human	WB	J Physiol Biochem; Rab11-FIP4 interacts with ARF5 to promote cancer stemness in hepatocellular carcinoma; Authors - Feifeng Song ; KD Validated