Anticorps Polyclonal de lapin anti-androgen receptor

• Phare
• Validé par KD/KO

androgen receptor Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

50

Avis (3)

N° de cat : 22089-1-AP

Synonymes

AR, androgen receptor,AR, NR3C4, DHTR

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Galerie de données de validation

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  WB analysis of MCF-7 using 22089-1-AP

  WB result of androgen receptor antibody (22089-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-androgen receptor transfected MCF-7 cells.

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  WB analysis using 22089-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22089-1-AP (androgen receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22089-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22089-1-AP (androgen receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 22089-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22089-1-AP (androgen receptor antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 22089-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22089-1-AP (androgen receptor antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human prostate cancer using 22089-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using androgen receptor antibody (22089-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human prostate cancer using 22089-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using androgen receptor antibody (22089-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human prostate cancer using 22089-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using androgen receptor antibody (22089-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of LNCaP using 22089-1-AP

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using androgen receptor antibody (22089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of LNCaP using 22089-1-AP

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using androgen receptor antibody (22089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of LNCaP using 22089-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using androgen receptor antibody (22089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HepG2, cellules MCF-7, cellules NIH/3T3, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer de la prostate humain
• Résultats positifs en IF/ICC: cellules LNCaP,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 38 publications below
• IHC: See 12 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

22089-1-AP cible androgen receptor dans les applications de WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: androgen receptor Protéine recombinante Ag17291
• Nom complet: androgen receptor
• Masse moléculaire calculée: 914 aa, 99 kDa
• Poids moléculaire observé: 75-80 kDa, 110-120 kDa
• Numéro d’acquisition GenBank: BC132975
• Symbole du gène: AR
• Identification du gène (NCBI): 367
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

1. What is the molecular weight of AR? Are there any isoforms of AR?

The molecular weight of full-length androgen receptor (AR-B) is 110 kDa. An additional variant, AR-A, has an 87 kDa size and lacks the N-terminal 187 amino acids of AR-A (PMID: 8108393). Recently, more splice variants of AR have been discovered, raising protein products of around 80 kDa length (PMID: 19244107), as well as an AR45 variant of 45 kDa size (PMID: 15634333). AR splice variants differ in their cell line-specific expression (PMID: 24570075).

2. What is the subcellular localization of AR?

AR can be present in either or both of the cytoplasm and nucleus. In androgen-deprived cells, AR is found predominantly in the cytoplasm, while stimulation by androgens causes enrichment of androgen-bound AR in the nucleus. AR shuttles between the cytoplasm and nucleus and its phosphorylation state has an impact on the subcellular localization (PMID: 16282370).

3. Is AR post-translationally modified?

Post-translational modifications of the AR include phosphorylation, acetylation, methylation, SUMOylation, and ubiquitination (PMID: 21820033). These modifications have an impact on receptor stability, activity, and can change the observed molecular weight of the AR.

4. How to study AR signaling in cell culture?

It is important to control levels of cell stimulation while also looking at AR signaling. Fetal bovine serum (FBS) that is typically used in cell culture contains low levels of testosterone that are enough to stimulate the growth of prostate cells (PMID: 19676093), including the LNCaP cell line that is a commonly used human prostatic carcinoma cell model (PMID: 6831420). One possibility for complete testosterone deprivation is to use charcoal stripped FBS that removes lipophilic agents, including androgens. It is also not recommended to use phenol red in your medium because it is a weak estrogen (PMID: 3458212). Cell stimulation is often conducted by dihydrotestosterone (DHT).

5. What is the role of AR in prostate cancer?

AR plays a key role in the development and physiology of the prostate gland, and also cancer progression (PMID: 15082523). Mutations in AR altering ligands have been observed. The progression of the prostate cancer depends on AR activity and therefore blocking AR activity or lowering androgen levels is a key step related to androgen deprivation therapy (ADT).

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; SMAD3 promotes expression and activity of the androgen receptor in prostate cancer; Authors - Hee-Young Jeon
human	WB	Research (Wash D C); The FTO Mediated N6-Methyladenosine Modification of DDIT4 Regulation with Tumorigenesis and Metastasis in Prostate Cancer; Authors - Yue Zhao
mouse	IHC	Oncogene; Identification of low-frequency variants of UGT1A3 associated with bladder cancer risk by next-generation sequencing.; Authors - Rui Zheng
human,mouse	WB,IHC	Biomed Pharmacother; Therapeutic targeting of histone lysine demethylase KDM4B blocks the growth of castration-resistant prostate cancer; Authors - Lingling Duan
mouse	IHC	Sci Total Environ; Imidacloprid disrupts the endocrine system by interacting with androgen receptor in male mice.; Authors - Xianling Yuan
mouse	WB,IF	Cancer Sci; Splicing factor PRPF6 upregulates oncogenic androgen receptor signaling pathway in hepatocellular carcinoma.; Authors - Huijuan Song

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Antonio (Verified Customer) (03-27-2023)

This antibody worked better than expected. Better than several Same antibody from different company

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000

FH

Emma (Verified Customer) (11-29-2021)

We couldn't get this antibody to work by IF on FFPE tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:20
• Cell Tissue Type: Prostate

FH

Saswati (Verified Customer) (08-20-2019)

This antibody worked in a cell line with a lower expression of AR, when another antibody from another vendor did not work. I am very pleased with its earlier lot.Also, since this is a polyclonal antibody, many bands light up, but in my opinion these are legitimate bands.Also hoping that the quality control keeps good eye from one lot to another., I have yet to use the particular lot# I put in this note.Thanks

• Applications: Western Blot, Immunofluorescence, Flow Cytometry,
• Primary Antibody Dilution: 1:6000
• Cell Tissue Type: MDA-MB 231

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