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Glucocorticoid receptor Polyclonal antibody

Glucocorticoid receptor Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 24050-1-AP

Synonyms

NR3C1, GRL, GR, GCR, GCCR



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, DU 145 cells, mouse brain tissue, rat brain tissue, PC-12 cells
Positive IP detected inHepG2 cells, mouse heart tissue
Positive IHC detected inhuman prostate cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24050-1-AP targets Glucocorticoid receptor in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish, rare minnow
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Glucocorticoid receptor fusion protein Ag21146
Full Name nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 94-97 kDa
GenBank Accession NumberBC015610
Gene Symbol Glucocorticoid receptor
Gene ID (NCBI) 2908
RRIDAB_2813890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).

Protocols

Product Specific Protocols
WB protocol for Glucocorticoid receptor antibody 24050-1-APDownload protocol
IHC protocol for Glucocorticoid receptor antibody 24050-1-APDownload protocol
IF protocol for Glucocorticoid receptor antibody 24050-1-APDownload protocol
IP protocol for Glucocorticoid receptor antibody 24050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

Individual-specific functional epigenomics reveals genetic determinants of adverse metabolic effects of glucocorticoids.

Authors - Wenxiang Hu
mouseIHC

Cell Metab

A macrophage-hepatocyte glucocorticoid receptor axis coordinates fasting ketogenesis.

Authors - Anne Loft
humanWB

Sci Adv

Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment.

Authors - Leiming Wang
mousechIP

Mol Cell

Cistromic Reprogramming of the Diurnal Glucocorticoid Hormone Response by High-Fat Diet.

Authors - Fabiana Quagliarini
mouseWB,ChIP,IP

J Clin Invest

REVERBa couples the circadian clock to hepatic glucocorticoid action.

Authors - Giorgio Caratti
mouseChIP

Mol Psychiatry

The co-chaperone Fkbp5 shapes the acute stress response in the paraventricular nucleus of the hypothalamus of male mice.

Authors - Alexander S Häusl

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Ping (Verified Customer) (06-04-2019)

This antibody works well for immunoprecipitation and western blot.

  • Applications: Western Blot, Immunoprecipitation,
  • Cell Tissue Type: mouse brain tissue
FH

Yu Siong (Verified Customer) (04-23-2019)

Multiple bands observed - Total of 5 bands, not suitable for porcine brain endothelial cells, as non-specific binding tend to occur.Consistent in two Independent experiments

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Porcine brain endothelial cells
FH

Praveen (Verified Customer) (02-25-2019)

WORKS VERY WELL.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Cell culture, ChIP,
  • Cell Tissue Type: Ac16
FH

Shubham (Verified Customer) (01-31-2019)

Antibody works great.

  • Applications: Western Blot, Immunohistochemistry, Cell culture,
  • Primary Antibody Dilution: 1:10
  • Cell Tissue Type: Ac16
Glucocorticoid receptor Antibody Western Blot,Immunohistochemistry,Cell culture, validation (1:10 dilution) in Ac16 (Cat no:24050-1-AP)