PR Polyclonal antibody

PR Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19980-1-AP

Synonyms

NR3C3, PGR, PR, progesterone receptor

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Validation Data Gallery

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  IHC staining of human breast cancer using 19980-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19980-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19980-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19980-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19980-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19980-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19980-1-AP (PR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 19980-1-AP

  Immunofluorescent analysis of MCF-7 cells using 19980-1-AP (PR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 19980-1-AP

  Immunofluorescent analysis of MCF-7 cells using 19980-1-AP (PR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19980-1-AP targets PR in IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: progesterone receptor
• Calculated Molecular Weight: 933 aa, 99 kDa
• GenBank Accession Number: NM_000926
• Gene Symbol: Progesterone Receptor
• Gene ID (NCBI): 5241
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGR, also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.