CYP19A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CYP19A1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 16554-1-AP

Synonyms

CYP19, CYAR, CPV1, Aromatase, ARO1

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Validation Data Gallery

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  WB analysis of A2780 using 16554-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 16554-1-AP (CYP19A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 16554-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16554-1-AP (CYP19A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 16554-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16554-1-AP (CYP19A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16554-1-AP (CYP19A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16554-1-AP (CYP19A1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16554-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16554-1-AP (CYP19A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of human placenta using 16554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human placenta using 16554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 16554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 16554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CYP19A1 antibody (16554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A2780 cells, mouse ovary tissue, human placenta tissue
• Positive IHC detected in: human placenta tissue, rat brain tissue, human kidney tissue, human skeletal muscle tissue, human gliomas tissue, human testis tissue, human spleen tissue, human lung tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human placenta tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

16554-1-AP targets CYP19A1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, geese
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP19A1 fusion protein Ag9765
• Full Name: cytochrome P450, family 19, subfamily A, polypeptide 1
• Calculated Molecular Weight: 20 kDa, 25 kDa, 58 kDa
• Observed Molecular Weight: 49-55 kDa
• GenBank Accession Number: BC022896
• Gene Symbol: CYP19A1
• Gene ID (NCBI): 1588
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP19A1(Cytochrome P450 19A1) is also named as ARO1(aromatase), CYAR, CYP19, estrogen synthase and belongs to the cytochrome P450 family. It is a terminal enzyme which transforms irreversibly androgens into estrogens and it is present in the endoplasmic reticulum of numerous tissues(PMID:16406261). The gene encodes a protein with the molecular weight between 46 kDa and 69 kDa(PMID:8129748) and the protein can be glycosylated, but the level of glycosylation does not seem to be essential for CYP19A1 activity(PMID:12606587). Brain aromatase may be neuroprotective by increasing the local estrogen levels in injured neurons so that genetic variation in the brain aromatase gene may modify the risk for AD(PMID:16767510).

Publications

Species	Application	Title
human	WB	Clin Transl Med; Treg deficiency-mediated TH 1 response causes human premature ovarian insufficiency through apoptosis and steroidogenesis dysfunction of granulosa cells.; Authors - Xue Jiao
mouse	WB	Sci Rep; Novel WT1 Missense Mutations in Han Chinese Women with Premature Ovarian Failure.; Authors - Huidan Wang
mouse, rat	WB	Sci Rep; Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application.; Authors - Jiansha Li ; KD Validated
human	WB,IHC	Fertil Steril; Elevated maternal androgen is associated with dysfunctional placenta and lipid disorder in newborns of mothers with polycystic ovary syndrome.; Authors - Miao Sun
rat	WB	Environ Pollut; Hormonal changes and folliculogenesis in female offspring of rats exposed to cadmium during gestation and lactation.; Authors - Zhiliang Li
mouse	WB	Endocrinology; The Immp2l Mutation Causes Ovarian Aging Through ROS-Wnt/β-Catenin-Estrogen Pathway: Preventive Effect of Melatonin.; Authors - Qing He