Anticorps Polyclonal de lapin anti-Aquaporin 4

• Phare
• Validé par KD/KO

Aquaporin 4 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

76

Avis (3)

N° de cat : 16473-1-AP

Synonymes

AQP4, AQP 4, Mercurial-insensitive water channel, Aquaporin-4, Aquaporin4

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Galerie de données de validation

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  WB analysis using 16473-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16473-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16473-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 16473-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16473-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 16473-1-AP

  IP result of anti-Aquaporin 4 (IP:16473-1-AP, 4ug; Detection:16473-1-AP 1:300) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16473-1-AP (Aquaporin 4 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16473-1-AP (Aquaporin 4 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of rat brain using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of mouse heart using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 16473-1-AP (Aquaporin 4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16473-1-AP (Aquaporin 4 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 16473-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16473-1-AP (Aquaporin 4 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of rat brain using 16473-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Aquaporin 4 antibody (16473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 16473-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Aquaporin 4 antibody (16473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), GFAP antibody (60190-1-Ig, Clone: 4B2E10, red).

• 

  IF Staining of mouse brain using 16473-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Aquaporin 4 antibody (16473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 16473-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Aquaporin 4 antibody (16473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), GFAP antibody (60190-1-Ig, Clone: 4B2E10, red).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules SKOV-3, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris, tissu rénal de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:2500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 47 publications below
• IHC: See 18 publications below
• IF: See 30 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

16473-1-AP cible Aquaporin 4 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Aquaporin 4 Protéine recombinante Ag9561
• Nom complet: aquaporin 4
• Masse moléculaire calculée: 323 aa, 35 kDa
• Poids moléculaire observé: 35-37 kDa, 32-34 kDa
• Numéro d’acquisition GenBank: BC022286
• Symbole du gène: AQP4
• Identification du gène (NCBI): 361
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

Publications

Species	Application	Title
human	IF	Clin Cancer Res; A Histopathologic Correlation Study Evaluating Glymphatic Function in Brain Tumors by Multi-Parametric MRI; Authors - Min Gao
human	IF	Brain; Frontal white matter hyperintensities, clasmatodendrosis and gliovascular abnormalities in ageing and post-stroke dementia.; Authors - Aiqing Chen
mouse	WB,IF	Brain Behav Immun; Polyunsaturated fatty acid supplement alleviates depression-incident cognitive dysfunction by protecting the cerebrovascular and glymphatic systems.; Authors - Xinghua Liu
mouse	WB	Glia; Haploinsufficiency of microglial MyD88 ameliorates Alzheimer's pathology and vascular disorders in APP/PS1-transgenic mice.; Authors - Wenqiang Quan
mouse	WB	Brain Behav Immun; Hypoxia augments LPS-induced inflammation and triggers high altitude cerebral edema in mice.; Authors - Yanzhao Zhou
mouse	IF	J Neuroinflammation; Regulatory T cells protect against brain damage by alleviating inflammatory response in neuromyelitis optica spectrum disorder.; Authors - Xue Ma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Paula (Verified Customer) (07-30-2024)

Worked well for human primary cell line, dilution used 1:500 (secondary 1:400)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human

Aquaporin 4 Antibody Immunofluorescence validation (1:500 dilution) in Human (Cat no:16473-1-AP)

FH

aidan (Verified Customer) (07-27-2024)

Very happy with this antibody would highly recommend. Overnight 4degrees, 1:2000. Bis tris gel. Secondary 1:4000 HRP 1h RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: muller retinal mouse

Aquaporin 4 Antibody Western Blot validation (1:2000 dilution) in muller retinal mouse (Cat no:16473-1-AP)

FH

Reyes (Verified Customer) (04-05-2024)

Aquaporin 4 (in green) shows a strong stainning, although difficult for the analyses, in human FFPE brain cortex.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

Aquaporin 4 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:16473-1-AP)

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