Kir4.1 Polyclonal antibody

Kir4.1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (2)

Cat no : 12503-1-AP

Synonyms

BIRK 10, KCNJ10, KCNJ13 PEN, KIR1.2, Kir4.1

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Validation Data Gallery

• 

  WB analysis using 12503-1-AP

  mouse brain tissues (heated at 100°C, unboided, heated at 37°C) were subjected to SDS PAGE followed by western blot with 12503-1-AP (Kir4.1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12503-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12503-1-AP (Kir4.1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 12503-1-AP

  IP result of anti-Kir4.1 (IP:12503-1-AP, 4ug; Detection:12503-1-AP 1:500) with rat brain tissue lysate 4800ug.

• 

  IHC staining of human gliomas using 12503-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12503-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12503-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 12503-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 12503-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 12503-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: human gliomas tissue, human brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 3 publications below

Product Information

12503-1-AP targets Kir4.1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Kir4.1 fusion protein Ag3182
• Full Name: potassium inwardly-rectifying channel, subfamily J, member 10
• Calculated Molecular Weight: 379 aa, 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC034036
• Gene Symbol: Kir4.1
• Gene ID (NCBI): 3766
• RRID: AB_2234144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Neuron; Glioma epileptiform activity and progression are driven by IGSF3-mediated potassium dysregulation; Authors - Rachel Naomi Curry
		Glia; Wnt/β-catenin signaling mediates the seizure-facilitating effect of postischemic reactive astrocytes after pentylenetetrazole-kindling.; Authors - Jialei Yang
mouse	IF	Glia; Elevated GFAP induces astrocyte dysfunction in caudal brain regions: A potential mechanism for hindbrain involved symptoms in type II Alexander disease.; Authors - Heather R Minkel
human	WB	Mult Scler; Low reliability of anti-KIR4.183-120 peptide auto-antibodies in multiple sclerosis patients.; Authors - Mariapaola Marino
mouse,rabbit	WB	Exp Eye Res; A novel model of subretinal edema induced by DL-alpha aminoadipic acid; Authors - Zhan Xie
rat	WB,IF	Am J Transl Res; Yigan Mingmu Decoction inhibits diabetic macular edema through regulating Kir4.1/AQP4 axis: a study based on network pharmacology; Authors - Longrong Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Paula (Verified Customer) (07-30-2024)

Worked well for human primary cell line, dilution used 1:500 (secondary 1:400)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human

Kir4.1 Antibody Immunofluorescence validation (1:500 dilution) in Human (Cat no:12503-1-AP)

FH

aidan (Verified Customer) (07-27-2024)

very good. would highly recommend

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: muller retinal mouse

Kir4.1 Antibody Western Blot validation (1:1000 dilution) in muller retinal mouse (Cat no:12503-1-AP)

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