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- KD/KO Validated
TRPV4 Polyclonal antibody
TRPV4 Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, ELISA
Conjugate
Unconjugated
Cat no : 20987-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| IHC | See 6 publications below |
Product Information
20987-1-AP targets TRPV4 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | transient receptor potential cation channel, subfamily V, member 4 |
| Calculated Molecular Weight | 98 kDa |
| GenBank Accession Number | NM_021625 |
| Gene Symbol | TRPV4 |
| Gene ID (NCBI) | 59341 |
| RRID | AB_10926386 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRPV4, also named as VRL2 and VROAC, belongs to the transient receptor family and TrpV subfamily. It is a non-selective calcium permeant cation channel protein which involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. It is activated by low pH, citrate and phorbol esters. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3), spondylometaphyseal dysplasia Kozlowski type (SMDK) and metatropic dysplasia (MTD). Multiple transcript variants encoding different isoforms have been found.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRPV4 antibody 20987-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4.
| ||
Cell Death Dis Inhibition of TRPV4 remodels single cell polarity and suppresses the metastasis of hepatocellular carcinoma | ||
Environ Pollut Exposure to both formaldehyde and high relative humidity exacerbates allergic asthma by activating the TRPV4-p38 MAPK pathway in Balb/c mice. | ||
Neuropharmacology TRPV4 inhibitor HC067047 produces antidepressant-like effect in LPS-induced depression mouse model. | ||
Cell Mol Bioeng The mechanosensitive ion channel TRPV4 is a regulator of lung development and pulmonary vasculature stabilization. |
