- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-ACC1
ACC1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (3)
Applications
WB, IHC, IF/ICC, IP, RIP, ELISA
Conjugaison
Non conjugué
129
N° de cat : 21923-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HEK-293, cellules HeLa, cellules HepG2, tissu cérébral de souris |
Résultats positifs en IP | cellules HepG2, |
Résultats positifs en IHC | tissu de muscle squelettique de souris, il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF/ICC | cellules HeLa, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochimie (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 6 publications below |
WB | See 119 publications below |
IHC | See 11 publications below |
IF | See 7 publications below |
IP | See 1 publications below |
RIP | See 1 publications below |
Informations sur le produit
21923-1-AP cible ACC1 dans les applications de WB, IHC, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | rat, bovin, Humain, porc, poulet, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | ACC1 Protéine recombinante Ag16452 |
Nom complet | acetyl-Coenzyme A carboxylase alpha |
Masse moléculaire calculée | 2383 aa, 275 kDa |
Poids moléculaire observé | 250 kDa |
Numéro d’acquisition GenBank | BC137287 |
Symbole du gène | Acetyl-CoA Carboxylase 1 |
Identification du gène (NCBI) | 31 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.
Protocole
Product Specific Protocols | |
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WB protocol for ACC1 antibody 21923-1-AP | Download protocol |
IHC protocol for ACC1 antibody 21923-1-AP | Download protocol |
IF protocol for ACC1 antibody 21923-1-AP | Download protocol |
IP protocol for ACC1 antibody 21923-1-AP | Download protocol |
FC protocol for ACC1 antibody 21923-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P | ||
Cell Metab Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism. | ||
Hepatology Acetyl-CoA carboxylase α promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients.
| ||
EMBO J TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis. | ||
EMBO J Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.
| ||
EMBO Rep Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kishor (Verified Customer) (02-22-2019) | I have got some good results with this Ab, but the band are not at the exact place (molecular weight) where it supposed to be
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