• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-ACC1

ACC1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugaison

Non conjugué

N° de cat : 21923-1-AP

Synonymes

ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1



Applications testées

Résultats positifs en WBcellules HEK-293, cellules HeLa, cellules HepG2, tissu cérébral de souris
Résultats positifs en IPcellules HepG2,
Résultats positifs en IHCtissu de muscle squelettique de souris,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HeLa,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

21923-1-AP cible ACC1 dans les applications de WB, IHC, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, bovin, Humain, porc, poulet, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène ACC1 Protéine recombinante Ag16452
Nom complet acetyl-Coenzyme A carboxylase alpha
Masse moléculaire calculée 2383 aa, 275 kDa
Poids moléculaire observé 250 kDa
Numéro d’acquisition GenBankBC137287
Symbole du gène Acetyl-CoA Carboxylase 1
Identification du gène (NCBI) 31
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.

Protocole

Product Specific Protocols
WB protocol for ACC1 antibody 21923-1-APDownload protocol
IHC protocol for ACC1 antibody 21923-1-APDownload protocol
IF protocol for ACC1 antibody 21923-1-APDownload protocol
IP protocol for ACC1 antibody 21923-1-APDownload protocol
FC protocol for ACC1 antibody 21923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P

Authors - Yuan-Li Ai
mouseWB

Cell Metab

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Authors - Yi Fu
humanWB

Hepatology

Acetyl-CoA carboxylase α promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients.

Authors - Ming-Da Wang
  • KD Validated
humanWB

EMBO J

TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.

Authors - Qichao Huang
mouseWB,IF

EMBO J

Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

Authors - Lihua Wang
  • KD Validated
humanWB

EMBO Rep

Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.

Authors - Yang Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kishor (Verified Customer) (02-22-2019)

I have got some good results with this Ab, but the band are not at the exact place (molecular weight) where it supposed to be

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
ACC1 Antibody Western Blot, validation (1:1000 dilution) in (Cat no:21923-1-AP)