|Positive WB detected in||HEK-293 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
16617-1-AP targets CAD in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CAD fusion protein Ag9912|
|Full Name||carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase|
|Calculated molecular weight||2225 aa, 243 kDa|
|Observed molecular weight||240 kDa|
|GenBank accession number||BC014178|
|Gene ID (NCBI)||790|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase) is a multifunctional enzyme required for the de novo synthesis of pyrimidine nucleotides (PMID:25422319). CAD initiates and controls the flflux through the pathway and is regulated by allosteric effectors and phosphorylations through different signaling cascades (PMID:12438317). Moreover, CAD is found upregulated in tumors, while mutations that compromise its function are the cause of a severe infantile epileptic disease (PMID:36677714).
|Product Specific Protocols|
|WB protocol for CAD antibody 16617-1-AP||Download protocol|
|IHC protocol for CAD antibody 16617-1-AP||Download protocol|
|IF protocol for CAD antibody 16617-1-AP||Download protocol|
|IP protocol for CAD antibody 16617-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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