• Featured Product
  • KD/KO Validated

BRCA1 Polyclonal antibody

BRCA1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 22362-1-AP

Synonyms

BRCA1, BRCAI, BRCC1, breast cancer 1, early onset, BROVCA1, IRIS, PSCP, RING finger protein 53, RNF53



Tested Applications

Positive WB detected inMCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue, rat testis tissue, mouse testis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

22362-1-AP targets BRCA1 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRCA1 fusion protein Ag17946
Full Name breast cancer 1, early onset
Calculated molecular weight 1863 aa, 208 kDa
Observed molecular weight 300 kDa
GenBank accession numberBC115037
Gene symbol BRCA1
Gene ID (NCBI) 672
RRIDAB_2879090
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRCA1, also named as RNF53, plays a central role in DNA repair by facilitating cellular response to DNA repair. It is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. It is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is required for FANCD2 targeting to sites of DNA damage. It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. The antibody is specific to BRCA1. BRCA1 appears to produce multiple splice variants. BRCA1 is a nuclear protein with a molecular mass of 220 kDa. The present study describes the isolation and expression of two cDNAs of BRCA1, including a splice variant designated BRCA1D672-4095. BRCA1D672-4095 is generated by exclusion of exon 11 by in-frame splicing and produces a 97 kDa protein. In contrast to BRCA1, BRCA1D672-4095 localizes to the cytoplasm.

Protocols

Product Specific Protocols
WB protocol for BRCA1 antibody 22362-1-APDownload protocol
IHC protocol for BRCA1 antibody 22362-1-APDownload protocol
IP protocol for BRCA1 antibody 22362-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.

Authors - Jiao Zhao
  • KD Validated
humanWB

Nat Commun

SIRT7 is a histone desuccinylase that functionally links to chromatin compaction and genome stability.

Authors - Lei Li
  • KD Validated
humanIF

Nucleic Acids Res

USP11 acts as a histone deubiquitinase functioning in chromatin reorganization during DNA repair.

Authors - Xia Ting

Oxid Med Cell Longev

Slowly Repaired Bulky DNA Damages Modulate Cellular Redox Environment Leading to Premature Senescence.

Authors - Yujie Zhang
humanWB

Elife

Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response.

Authors - Kerrie-Ann McMahon
humanWB,IHC

Oncogene

PTEN deficiency sensitizes endometrioid endometrial cancer to compound PARP-PI3K inhibition but not PARP inhibition as monotherapy.

Authors - X Bian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hasan (Verified Customer) (08-08-2022)

Western blot for HeLa cells transfected with siRNA against BRCA1 or control. anti-BRCA1 Ab (22362-1-AP) was used at dilution of (1:2000) in 5% skimmed milk. incubation overnight at 4 degrees.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HeLa cells
BRCA1 Antibody Western Blot validation (1:2000 dilution) in HeLa cells (Cat no:22362-1-AP)