WTAP Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

WTAP Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, RIP, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

65

CloneNo.

4A10G9

Rezensionen (2)

Kat-Nr. : 60188-1-Ig

Synonyme

Female lethal(2)D homolog, hFL(2)D, KIAA0105, WT1 associated protein, WTAP

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Galerie der Validierungsdaten

• 

  WB analysis using 60188-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60188-1-Ig (WTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60188-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60188-1-Ig (WTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  IHC staining of human colon using 60188-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60188-1-Ig (WTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60188-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60188-1-Ig (WTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 60188-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 60188-1-Ig(WTAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, LNCaP-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 55 publications below
• IHC: See 11 publications below
• IF: See 6 publications below
• IP: See 4 publications below
• FC: See 1 publications below
• RIP: See 1 publications below

Produktinformation

60188-1-Ig bindet in WB, RIP, IP, IHC, IF, FC, ELISA WTAP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, drosophila, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: WTAP fusion protein Ag0259
• Vollständiger Name: Wilms tumor 1 associated protein
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC000383
• Gene symbol: WTAP
• Gene ID (NCBI): 9589
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 45-50 kDa due to phosphorylation.

Publikationen

Species	Application	Title
mouse	WB	Science; Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.; Authors - Shay Geula
human	WB	Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
mouse	IHC, IF	Nat Neurosci; Synaptic N6-methyladenosine (m6A) epitranscriptome reveals functional partitioning of localized transcripts.; Authors - Daria Merkurjev
human	WB,IP	Mol Cell; Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases m 6 A Methylation; Authors - Hui-Lung Sun
human	WB	Mol Cell; Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection.; Authors - Nandan S Gokhale
human	WB	Mol Cell; mTORC1 promotes cell growth via m6A-dependent mRNA degradation.; Authors - Sungyun Cho ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elaine (Verified Customer) (01-20-2021)

Antibody works fine in Western blot on MEF cells

• Applications: Western Blot

FH

Daniel (Verified Customer) (06-25-2019)

RCC4 grown in normoxic (left lane) and hypoxic (right lane) conditions.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RCC4 human cells

WTAP Antibody Western Blot, validation (1:2000 dilution) in RCC4 human cells (Cat no:60188-1-Ig)

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