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Cyclin A2 Polyclonal antibody
Cyclin A2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 18202-1-AP
Validation Data Gallery
|Positive WB detected in||HL-60 cells, HeLa cells, U-937 cells, NIH/3T3 cells, MCF-7 cells, K-562 cells|
|Positive IP detected in||HL-60 cells|
|Positive IHC detected in||human tonsillitis tissue, human breast cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
18202-1-AP targets Cyclin A2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cyclin A2 fusion protein Ag12765|
|Full Name||cyclin A2|
|Calculated molecular weight||432 aa, 49 kDa|
|Observed molecular weight||48-52 kDa|
|GenBank accession number||BC104783|
|Gene ID (NCBI)||890|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.
|Product Specific Protocols|
|WB protocol for Cyclin A2 antibody 18202-1-AP||Download protocol|
|IHC protocol for Cyclin A2 antibody 18202-1-AP||Download protocol|
|IF protocol for Cyclin A2 antibody 18202-1-AP||Download protocol|
|IP protocol for Cyclin A2 antibody 18202-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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