Cyclin A2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Cyclin A2 Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

181

Cat no : 18202-1-AP

Synonyms

CCNA2, CyclinA2, Cyclin-A, Cyclin A, CCNA

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Validation Data Gallery

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  WB analysis using 18202-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18202-1-AP (Cyclin A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18202-1-AP

  WB result of Cyclin A2 antibody (18202-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin A2 transfected HeLa cells.

• 

  WB analysis using 18202-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18202-1-AP (Cyclin A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18202-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18202-1-AP (Cyclin A2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 18202-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18202-1-AP (CCNA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 18202-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18202-1-AP (Cyclin A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 18202-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 18202-1-AP (Cyclin A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 18202-1-AP

  IP result of anti-Cyclin A2 (IP:18202-1-AP, 4ug; Detection:18202-1-AP 1:20000) with HeLa cells lysate 1320 ug.

• 

  IP experiment of HL-60 using 18202-1-AP

  IP result of anti-Cyclin A2 (IP:18202-1-AP, 3ug; Detection:18202-1-AP 1:500) with HL-60 cells lysate 4000ug.

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  IHC staining of human tonsillitis using 18202-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18202-1-AP (Cyclin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 18202-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18202-1-AP (Cyclin A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18202-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18202-1-AP (Cyclin A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18202-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18202-1-AP (Cyclin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18202-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18202-1-AP (Cyclin A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18202-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18202-1-AP (Cyclin A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18202-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cyclin A2 antibody (18202-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of MCF-7 using 18202-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin A2 antibody (18202-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 18202-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclin A2 (18202-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive WB detected in: HL-60 cells, K-562 cells, U-937 cells, HeLa cells, NIH/3T3 cells, MCF-7 cells
• Positive IP detected in: HeLa cells, HL-60 cells
• Positive IHC detected in: human tonsillitis tissue, human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, MCF-7 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 173 publications below
• CoIP: See 1 publications below

Product Information

18202-1-AP targets Cyclin A2 in WB, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclin A2 fusion protein Ag12765
• Full Name: cyclin A2
• Calculated Molecular Weight: 432 aa, 49 kDa
• Observed Molecular Weight: 48-52 kDa
• GenBank Accession Number: BC104783
• Gene Symbol: Cyclin A2
• Gene ID (NCBI): 890
• RRID: AB_10597084
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.

Publications

Species	Application	Title
rat	WB	Cell Metab; Sphingolipid metabolism controls mammalian heart regeneration; Authors - Xiaoqian Ji
mouse	WB	Nat Metab; LIFR regulates cholesterol-driven bidirectional hepatocyte-neutrophil cross-talk to promote liver regeneration; Authors - Yalan Deng
		Mol Cell; A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis; Authors - Kaiwen Bao
human	WB	Mol Cancer; CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.; Authors - Bing Yao
mouse		Acta Pharm Sin B; The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.; Authors - Weimin Yu
human	WB	Sci Adv; The p23 co-chaperone is a succinate-activated COX-2 transcription factor in lung adenocarcinoma tumorigenesis; Authors - Zhenlong Yu

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