WT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

WT1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IF/ICC, FC (Intra), IP, chIP, ELISA

Conjugate

Unconjugated

Publications

43

Reviews (2)

Cat no : 12609-1-AP

Synonyms

Wilms tumor protein, Wilms tumor 1

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Validation Data Gallery

• 

  WB analysis of K-562 using 12609-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12609-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 12609-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse kidney using 12609-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of human liver using 12609-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12609-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 12609-1-AP

  IP result of anti-WT1 (IP:12609-1-AP, 4ug; Detection:12609-1-AP 1:1000) with K-562 cells lysate 1360 ug.

• 

  IF Staining of K-562 using 12609-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using WT1 antibody (12609-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of K-562 using 12609-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human WT1 (12609-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: K-562 cells, A431 cells, human liver tissue, MCF-7 cells, mouse kidney tissue, rat kidney tissue
• Positive IP detected in: K-562 cells
• Positive IF/ICC detected in: K-562 cells
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 23 publications below
• IF: See 16 publications below
• ChIP: See 4 publications below

Product Information

12609-1-AP targets WT1 in WB, IF/ICC, FC (Intra), IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, bovine, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WT1 fusion protein Ag3337
• Full Name: Wilms tumor 1
• Calculated Molecular Weight: 449 aa, 49 kDa, 57 kDa
• Observed Molecular Weight: 52-55 kDa
• GenBank Accession Number: BC032861
• Gene Symbol: WT1
• Gene ID (NCBI): 7490
• RRID: AB_2216225
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. Function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. WT1 exists some isoforms with the range of molecular weight is 33-50 kDa.

Publications

Species	Application	Title
human	WB	Mol Cell; The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma.; Authors - Michael Heider
mouse	WB,IF	Mol Psychiatry; Brain-specific Wt1 deletion leads to depressive-like behaviors in mice via the recruitment of Tet2 to modulate Epo expression.; Authors - Fen Ji ; KO Validated
mouse	chIP	EMBO Mol Med; Inhibition of Aurora Kinase B attenuates fibroblast activation and pulmonary fibrosis.; Authors - Rajesh K Kasam
human	WB	Oncogene; YAP induces high-grade serous carcinoma in fallopian tube secretory epithelial cells.; Authors - G Hua
mouse	WB	Oncogene; USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model.; Authors - Juntae Kwon
human	IF	Elife; Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4.; Authors - Jianlin Liang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sandrine (Verified Customer) (12-17-2021)

clean signal for IP and western blot. Good relative enrichment for ChIP assays.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation, ChIP
• Cell Tissue Type: kidney tissue/Podocytes

FH

K (Verified Customer) (12-20-2020)

This Ab worked well for IHC (1:200) but I could't get satisfactory results for western blotting.

• Applications: Western Blot, Immunohistochemistry
• Cell Tissue Type: mouse liver tissues. rat liver and human liver cells

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