Validation Data Gallery
|Positive WB detected in||K-562 cells, human liver tissue, rat kidney tissue, mouse kidney tissue, A431 cells, MCF-7 cells|
|Positive IF detected in||K-562 cells|
|Positive FC detected in||K-562 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12609-1-AP targets WT1 in WB, IF, FC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, goat, chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||WT1 fusion protein Ag3337|
|Full Name||Wilms tumor 1|
|Calculated molecular weight||449 aa, 49 kDa, 57 kDa|
|Observed molecular weight||52-55 kDa|
|GenBank accession number||BC032861|
|Gene ID (NCBI)||7490|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. Function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. WT1 exists some isoforms with the range of molecular weight is 33-50 kDa.
The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma.
Brain-specific Wt1 deletion leads to depressive-like behaviors in mice via the recruitment of Tet2 to modulate Epo expression.
EMBO Mol Med
Inhibition of Aurora Kinase B attenuates fibroblast activation and pulmonary fibrosis.
YAP induces high-grade serous carcinoma in fallopian tube secretory epithelial cells.
USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model.
Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sandrine (Verified Customer) (12-17-2021)
clean signal for IP and western blot. Good relative enrichment for ChIP assays.
K (Verified Customer) (12-20-2020)
This Ab worked well for IHC (1:200) but I could't get satisfactory results for western blotting.