|Positive WB detected in||MCF-7 cells, Jurkat cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Sample-dependent, check data in validation data gallery|
The immunogen of 26754-1-AP is CENPA Fusion Protein expressed in E. coli.
|Host / Isotype||Rabbit / IgG|
|Immunogen||CENPA fusion protein Ag25025|
|Full Name||centromere protein A|
|Calculated molecular weight||16 kDa|
|Observed molecular weight||16 kDa|
|GenBank accession number||BC002703|
|Gene ID (NCBI)||1058|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Centromere protein-A (CENP-A) is a Histone H3-like protein that contains a C-terminal H3-like domain, required for centro-mere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA, CENP-A forms an octameric complex with histones H4, H2A and H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.
Five-gene signature associating with Gleason score serve as novel biomarkers for identifying early recurring events and contributing to early diagnosis for Prostate Adenocarcinoma.
A pan-cancer landscape of centromere proteins in tumorigenesis and anticancer drug sensitivity