CENPA Polyclonal antibody

CENPA Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26754-1-AP

Synonyms

CENP A, CENPA, Centromere autoantigen A, centromere protein A

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Validation Data Gallery

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  WB analysis of MCF-7 using 26754-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26754-1-AP (CENPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 26754-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26754-1-AP (CENPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MCF-7 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

Sample-dependent, check data in validation data gallery

Published Applications

• IHC: See 2 publications below

Product Information

The immunogen of 26754-1-AP is CENPA Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CENPA fusion protein Ag25025
• Full Name: centromere protein A
• Calculated molecular weight: 16 kDa
• Observed molecular weight: 16 kDa
• GenBank accession number: BC002703
• Gene symbol: CENPA
• Gene ID (NCBI): 1058
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Centromere protein-A (CENP-A) is a Histone H3-like protein that contains a C-terminal H3-like domain, required for centro-mere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA, CENP-A forms an octameric complex with histones H4, H2A and H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.

Publications

Species	Application	Title
human	IHC	J Cancer; Five-gene signature associating with Gleason score serve as novel biomarkers for identifying early recurring events and contributing to early diagnosis for Prostate Adenocarcinoma.; Authors - Lingyu Zhang
human	IHC	Transl Oncol; A pan-cancer landscape of centromere proteins in tumorigenesis and anticancer drug sensitivity; Authors - Yuan-Xiang Shi