CENPA Polyclonal antibody

CENPA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 26754-1-AP

Synonyms

Histone H3-like centromeric protein A, CENP-A, CENP A

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Validation Data Gallery

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  WB analysis of MCF-7 using 26754-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26754-1-AP (CENPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 26754-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26754-1-AP (CENPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 26754-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 26754-1-AP (CENPA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, Jurkat cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

26754-1-AP targets CENPA in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CENPA fusion protein Ag25025
• Full Name: centromere protein A
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16-20 kDa
• GenBank Accession Number: BC002703
• Gene Symbol: CENPA
• Gene ID (NCBI): 1058
• RRID: AB_2880622
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Centromere protein-A (CENP-A) is a Histone H3-like protein that contains a C-terminal H3-like domain, required for centro-mere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA, CENP-A forms an octameric complex with histones H4, H2A and H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.

Publications

Species	Application	Title
human	IHC	J Cancer; Five-gene signature associating with Gleason score serve as novel biomarkers for identifying early recurring events and contributing to early diagnosis for Prostate Adenocarcinoma.; Authors - Lingyu Zhang
human	IHC	Transl Oncol; A pan-cancer landscape of centromere proteins in tumorigenesis and anticancer drug sensitivity; Authors - Yuan-Xiang Shi
human	WB	Pathol Res Pract; CENPA facilitates glioma stem cell stemness and suppress ferroptosis to accelerate glioblastoma multiforme progression by promoting GBP2 transcription; Authors - Chuankun Li