GPR132 Polyclonal antibody

• Featured Product
• KD/KO Validated

GPR132 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 17026-1-AP

Synonyms

G protein coupled receptor 132, G2 accumulation protein, G2A, GPR132

---

Validation Data Gallery

• 

  IHC staining of human kidney using 17026-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17026-1-AP (GPR132 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 17026-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17026-1-AP (GPR132 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17026-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17026-1-AP (GPR132 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17026-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17026-1-AP (GPR132 antibody) at dilution of 1:100 (under 40x lens).

---

Tested Applications

• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• IHC: See 1 publications below

Product Information

17026-1-AP targets GPR132 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR132 fusion protein Ag10325
• Full Name: G protein-coupled receptor 132
• Calculated molecular weight: 380 aa, 43 kDa
• GenBank accession number: BC084546
• Gene symbol: GPR132
• Gene ID (NCBI): 29933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR132, also known as G2A (G2 accumulation protein), belongs to the G-protein coupled receptor (GPCR) 1 family. GPR132 is a DNA damage and stress inducible GPCR that blocks cells in G2/M. It functions at the G2/M checkpoint to delay mitosis and may serve as a mechanism for T and B cells and other cell types to slow their proliferation and repair damaged DNA to ensure proper replication (PMID: 9770487). Lysophosphatidylcholine (LPC) is a high affinity ligand for GPR132 (PMID: 15653487).

Publications

Species	Application	Title
human	IHC	J Eur Acad Dermatol Venereol; Lysophosphatidylcholine facilitates the pathogenesis of psoriasis through activating keratinocytes and T cells differentiation via glycolysis; Authors - Panpan Liu
mouse		Front Oncol; Lactate increases tumor malignancy by promoting tumor small extracellular vesicles production via the GPR81-cAMP-PKA-HIF-1α axis; Authors - Man Luo ; KD Validated
mouse		Neurosci Res; Proton-sensing receptor GPR132 facilitates migration of astrocytes.; Authors - Ken Matoba
human		J Hepatobiliary Pancreat Sci; Partial characterization of proapoptotic action of biliary deteriorated lipids on biliary epithelial cells in pancreaticobiliary diseases.; Authors - Fujita Keiko K
mouse		Nat Commun; Lysophosphatidylserine induces necrosis in pressure overloaded male mouse hearts via G protein coupled receptor 34; Authors - Ryuta Sugihara