Beta Tubulin Polyklonaler Antikörper

Beta Tubulin Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (6)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

380

Kat-Nr. : 10068-1-AP

Synonyme

Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain

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• Galerie der Validierungsdaten
• Proteintech vs. Other Vendors

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  WB analysis using 10068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10068-1-AP (Beta Tubulin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10068-1-AP (beta Tubulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10068-1-AP

  IP result of anti-Beta Tubulin (IP:10068-1-AP, 4ug; Detection:10068-1-AP 1:5000) with HEK-293 cells lysate 2320 ug.

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  IHC staining of mouse brain using 10068-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10068-1-AP (beta Tubulin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 10068-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10068-1-AP (beta Tubulin antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 10068-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10068-1-AP (beta Tubulin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 10068-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10068-1-AP (beta Tubulin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat brain using 10068-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10068-1-AP (beta Tubulin antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat brain using 10068-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10068-1-AP (beta Tubulin antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of C2C12 using 10068-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Beta Tubulin antibody (10068-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 10068-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10068-1-AP (beta Tubulin antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10068-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10068-1-AP (beta Tubulin antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10068-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Beta Tubulin (10068-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: C2C12-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 353 publications below
• IHC: See 2 publications below
• IF: See 12 publications below
• IP: See 1 publications below

Produktinformation

10068-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA Beta Tubulin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Caenorhabditis elegans, Hausschwein, Hund, Maus, Ratte, Rind, Zebrafisch, Seidenraupe
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Beta Tubulin fusion protein Ag0117
• Vollständiger Name: tubulin, beta 3
• Berechnetes Molekulargewicht: 450 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC000748
• Gene symbol: TUBB3
• Gene ID (NCBI): 10381
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

Publikationen

Species	Application	Title
human	WB	Cell; Complement Signals Determine Opposite Effects of B Cells in Chemotherapy-Induced Immunity.; Authors - Yiwen Lu
human	WB	Nat Struct Mol Biol; CRISPR-Cas9-based functional interrogation of unconventional translatome reveals human cancer dependency on cryptic non-canonical open reading frames; Authors - Caishang Zheng
	WB	Nat Commun; Gonococcal OMV-delivered PorB induces epithelial cell mitophagy; Authors - Shuai Gao
	WB	Adv Sci (Weinh); EGFR-Induced and c-Src-Mediated CD47 Phosphorylation Inhibits TRIM21-Dependent Polyubiquitylation and Degradation of CD47 to Promote Tumor Immune Evasion; Authors - Linyong Du
mouse	WB	Acta Pharm Sin B; Nuciferine protects against high-fat diet-induced hepatic steatosis and insulin resistance via activating TFEB-mediated autophagy-lysosomal pathway.; Authors - Xiliang Du
rat	WB	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu

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