p63 Monoklonaler Antikörper

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• KD/KO Validated

p63 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

2B6B2

Kat-Nr. : 60332-1-Ig

Synonyme

AIS, B(p51A), B(p51B), CUSP, deltaNp63, EEC3, KET, LMS, NBP, OFC8, p40, p51, p53CP, p63, p73H, p73L, RHS, SHFM4, TP53CP, TP53L, TP63, TP73L, Tumor protein 63, tumor protein p63, Tumor protein p73 like

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 60332-1-Ig

  WB result of TP63 antibody (60332-1-Ig, 1:1000) with si-control and si-TP63 transfected HepG2 cells.

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  WB analysis of PC-3 using 60332-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60332-1-Ig (TP63 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 60332-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-1-Ig (TP63 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 60332-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-1-Ig (TP63 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HeLa
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

60332-1-Ig bindet in WB, IHC, ELISA p63 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: p63 fusion protein Ag2791
• Vollständiger Name: tumor protein p63
• Berechnetes Molekulargewicht: 680 aa, 77 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC039815
• Gene symbol: TP63
• Gene ID (NCBI): 8626
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L). It is Nuclear stain.

Publikationen

Species	Application	Title
human	IHC	Nat Commun; Interplay and cooperation between SREBF1 and master transcription factors regulate lipid metabolism and tumor-promoting pathways in squamous cancer.; Authors - Li-Yan Li
human	IHC	Andrologia; Circ_0076305 facilitates prostate cancer development via sponging miR-411-5p and regulating PGK1.; Authors - Xiqi Ding

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