Thrombomodulin Polyklonaler Antikörper

Thrombomodulin Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 14318-1-AP

Synonyme

CD141, Fetomodulin, THBD, THRM, thrombomodulin, TM

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Galerie der Validierungsdaten

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  WB analysis using 14318-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14318-1-AP (Thrombomodulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14318-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14318-1-AP (Thrombomodulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14318-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14318-1-AP (Thrombomodulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 14318-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14318-1-AP (Thrombomodulin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 14318-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14318-1-AP (Thrombomodulin antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of A431 using 14318-1-AP

  1X10^6 A431 cells were stained with .2ug Thrombomodulin antibody (14318-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, humanes Herzgewebe, THP-1-Zellen
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

14318-1-AP bindet in WB, IHC, IF, FC, ELISA Thrombomodulin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Thrombomodulin fusion protein Ag5558
• Vollständiger Name: thrombomodulin
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC035602
• Gene symbol: THBD
• Gene ID (NCBI): 7056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).

Publikationen

Species	Application	Title
rat	IF	Bioengineered; The crescendo pulse frequency of shear stress stimulates the endothelialization of bone marrow mesenchymal stem cells on the luminal surface of decellularized scaffold in the bioreactor; Authors - Yuhao Jiao
human	WB	Front Cell Dev Biol; Crocin Improves Endothelial Mitochondrial Dysfunction via GPx1/ROS/KCa3.1 Signal Axis in Diabetes.; Authors - Xuemei Li
human	IHC	Diagnostics (Basel); Urinary SARS-CoV-2 RNA Is an Indicator for the Progression and Prognosis of COVID-19.; Authors - Lu Zhang
human	IHC	Interact Cardiovasc Thorac Surg; Atrial endocardial expression of von Willebrand factor and thrombomodulin is associated with recurrence after minimally invasive surgical atrial fibrillation ablation.; Authors - Kang An
human	WB,IF	Ther Apher Dial; Decreased Expression of Thrombomodulin in Endothelial Cells by Fibroblast Growth Factor-23/α-Klotho.; Authors - Kenji Tanaka
human	IHC	Clin Appl Thromb Hemost; Endocardial Changes in Nonvalvular Atrial Fibrillation Without Atrial Thrombus-Thrombomodulin and Tissue Factor Pathway Inhibitor.; Authors - Kang An

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