Validation Data Gallery
|Positive WB detected in||A431 cells, HepG2 cells, HeLa cells, MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue, mouse cerebellum tissue, mouse spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||U-251 cells, HeLa cells, HEK-293 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10801-1-AP targets PSAP in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PSAP fusion protein Ag1194|
|Calculated molecular weight||58 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC001503|
|Gene ID (NCBI)||5660|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The PSAP gene encodes prosaposin, a precursor of four small nonenzymatic glycoproteins termed 'sphingolipid activator proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A is encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. Saposins A-D are required for the hydrolysis of certain sphingolipids by specific lysosomal hydrolases. (PMID: 2001789) Defects in PSAP are the cause of Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy (PubMed: 2060627, PMID: 15773042). This PSAP antibody (10801-1-AP) is expected to recognize both saposin A and B.
Genome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosis.
Adv Sci (Weinh)
Integrative Transcriptomic Analyses of Hippocampal-Entorhinal System Subfields Identify Key Regulators in Alzheimer's Disease
Prosaposin is a regulator of progranulin levels and oligomerization.
Impaired prosaposin lysosomal trafficking in frontotemporal lobar degeneration due to progranulin mutations.
Accumulation of saposin in dystrophic neurites is linked to impaired lysosomal functions in Alzheimer's disease brains.
Proc Natl Acad Sci U S A
Inhibition of sphingolipid synthesis improves outcomes and survival in GARP mutant wobbler mice, a model of motor neuron degeneration.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sharan (Verified Customer) (05-28-2020)
The antibody works well in western blot assays with minimum cross-reactivity
Krishnendu (Verified Customer) (02-27-2020)
Very specific for AIMP3