F5 Polyclonal antibody

F5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20963-1-AP

Synonyms

Activated protein C cofactor, Coagulation factor V, F5, FVL, PCCF, Proaccelerin, labile factor

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Validation Data Gallery

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  WB analysis of mouse liver using 20963-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20963-1-AP (F5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20963-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20963-1-AP (F5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 20963-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20963-1-AP (F5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 20963-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20963-1-AP (F5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

20963-1-AP targets F5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: coagulation factor V (proaccelerin, labile factor)
• Calculated Molecular Weight: 252 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: NM_000130
• Gene Symbol: F5
• Gene ID (NCBI): 2153
• RRID: AB_2878773
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

There are 13 blood coagulation factors found in the blood. There are 13 blood coagulation factors found in the blood. F5 has little or no intrinsic procoagulant activity prior to its activation through limited proteolysis by thrombin or F10a at Arg709, Arg1018 and Arg1545. The resulting F5a molecule is a heterodimer that consists of a 105 kDa heavy chain and a 71/74 kDa light chain that are noncovalently associated in a calcium-dependent manner. The intact procofactor molecule (330 kDa) as well as proteins similar in size to the factor Va light chain (74 kDa) and both light chain precursors (280 and 220 kDa) found in factor Va(IIa) were observed. And factor Xa cleaves the factor Va light chain at Arg1765 into fragments of ~30 and ~48 kDa (residues 1546-1765 and 1766-2196, respectively) in the bovine and human systems.

Publications

Species	Application	Title
human	IHC	Cancers (Basel); The Immunological Contribution of a Novel Metabolism-Related Signature to the Prognosis and Anti-Tumor Immunity in Cervical Cancer.; Authors - Sihui Yu
human	IHC	Thyroid; Clinical and Molecular Characterizations of Papillary Thyroid Cancer in Children and Young Adults: a Multicenter Retrospective Study.; Authors - Kai Guo