• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

SMN Polyklonaler Antikörper

SMN Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22329-1-AP

Synonyme

C BCD541, Component of gems 1, Gemin 1, SMN, SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using SMN Polyclonal antibody (22329-1-AP)

    WB analysis of HEK-293 using 22329-1-AP

    WB result of SMN antibody (22329-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN transfected HEK-293 cells.

  • Western Blot (WB) analysis of K-562 cells using SMN Polyclonal antibody (22329-1-AP)

    WB analysis of K-562 using 22329-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 22329-1-AP (SMN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SMN Polyclonal antibody (22329-1-AP)

    WB analysis of HepG2 using 22329-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 22329-1-AP (SMN antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SMN Polyclonal antibody (22329-1-AP)

    WB analysis of HEK-293 using 22329-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22329-1-AP (SMN antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SMN Polyclonal antibody (22329-1-AP)

    WB analysis of Jurkat using 22329-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22329-1-AP (SMN antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using SMN Polyclonal antibody (22329-1-AP)

    IHC staining of human testis using 22329-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22329-1-AP (SMN antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SMN Polyclonal antibody (22329-1-AP)

    IHC staining of human testis using 22329-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22329-1-AP (SMN antibody at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SMN Polyclonal antibody (22329-1-AP)

    IF Staining of HepG2 using 22329-1-AP

    Immunofluorescent analysis of HepG2 cells using 22329-1-AP (SMN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBK-562-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen
Erfolgreiche Detektion in IHChumanes Hodengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

22329-1-AP bindet in WB, IHC, IF,ELISA SMN und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SMN fusion protein Ag17798
Vollständiger Name survival of motor neuron 2, centromeric
Berechnetes Molekulargewicht 282 aa, 30 kDa
Beobachtetes Molekulargewicht 38 kDa
GenBank-ZugangsnummerBC000908
Gene symbol SMN2
Gene ID (NCBI) 6607
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-affinitätsgereinigt
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA.

Protokolle

Produktspezifische Protokolle
WB protocol for SMN antibody 22329-1-APProtokoll herunterladen
IHC protocol for SMN antibody 22329-1-APProtokoll herunterladen
IF protocol for SMN antibody 22329-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen