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SMARCA4/BRG1 Polyklonaler Antikörper

SMARCA4/BRG1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21634-1-AP

Synonyme

ATP dependent helicase SMARCA4, BAF190, BAF190A, BRG1, BRG1 associated factor 190A, hSNF2b, Protein brahma homolog 1, Protein BRG 1, SMARCA4, SMARCA4/BRG1, SNF2, SNF2 BETA, SNF2B, SNF2L4, SNF2LB, SWI2, Transcription activator BRG1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, Maushirngewebe, MCF-7-Zellen, PC-3-Zellen, Rattenhirngewebe
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Kolonkarzinomgewebe, humanes Gliomgewebe, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe, Mausnierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFHepG2-Zellen, HEK-293-Zellen, HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:3000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:200-1:800
Immunfluoreszenz (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

21634-1-AP bindet in WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA SMARCA4/BRG1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Rind, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SMARCA4/BRG1 fusion protein Ag16256
Vollständiger Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Berechnetes Molekulargewicht 1647 aa, 185 kDa
Beobachtetes Molekulargewicht 185 kDa
GenBank-ZugangsnummerBC150298
Gene symbol SMARCA4
Gene ID (NCBI) 6597
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Protokolle

Produktspezifische Protokolle
WB protocol for SMARCA4/BRG1 antibody 21634-1-APProtokoll herunterladen
IHC protocol for SMARCA4/BRG1 antibody 21634-1-APProtokoll herunterladen
IF protocol for SMARCA4/BRG1 antibody 21634-1-APProtokoll herunterladen
IP protocol for SMARCA4/BRG1 antibody 21634-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIHC

J Clin Oncol

Genomic Characterization of Non-Small-Cell Lung Cancer in African Americans by Targeted Massively Parallel Sequencing.

Authors - Luiz H Araujo
humanWB

Cell Stem Cell

The long noncoding RNA lncTCF7 promotes self-renewal of human liver cancer stem cells through activation of Wnt signaling.

Authors - Yanying Wang
ChIP

Mol Cell

SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.

Authors - Shibin Hu
humanWB,IP

Nucleic Acids Res

Reactivation of the G1 enhancer landscape underlies core circuitry addiction to SWI/SNF

Authors - Katerina Cermakova
humanWB,IHC

Nat Commun

LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells.

Authors - Pingping Zhu
mouseCoIP

Nucleic Acids Res

HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.

Authors - Xu Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Satyendra (Verified Customer) (07-16-2021)

I have marked Knock out as KO

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK 293
SMARCA4/BRG1 Antibody Western Blot validation (1:1000 dilution) in HEK 293 (Cat no:21634-1-AP)