SMARCB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SMARCB1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 20654-1-AP

Synonyms

BAF47, BRG1 associated factor 47, hSNF5, hSNFS, INI1, INI-1, Integrase interactor 1 protein, RDT, Sfh1p, SMARCB1, SNF5, SNF5 homolog, SNF5L1, Snr1

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Validation Data Gallery

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  WB analysis of HepG2 using 20654-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20654-1-AP (SMARCB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20654-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20654-1-AP (SMARCB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 20654-1-AP

  IP result of anti-SMARCB1 (IP:20654-1-AP, 4ug; Detection:20654-1-AP 1:500) with K-562 cells lysate 3200ug.

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  IHC staining of human lymphoma using 20654-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 20654-1-AP (SMARCB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 20654-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20654-1-AP (SMARCB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 20654-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20654-1-AP (SMARCB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 20654-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 20654-1-AP (SMARCB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human lymphoma tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

20654-1-AP targets SMARCB1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: NM_003073
• Gene Symbol: SMARCB1
• Gene ID (NCBI): 6598
• RRID: AB_10695761
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCB1, also named as BAF47, INI1 and SNF5L1, belongs to the SNF5 family. It is a core component of the BAF (hSWI/SNF) complex. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. SMARCB1 stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. It is involved in activation of CSF1 promoter. SMARCB1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. SMARCB1 plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Defects in SMARCB1 are a cause of rhabdoid tumor (RDT) which also known as malignant rhabdoid tumor (MRT). Defects in SMARCB1 are a cause of schwannomatosis. The antibody is specific to SMARCB1.

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.; Authors - Li Wang
human	WB	Front Cell Dev Biol; Upregulation of Neogenin-1 by a CREB1-BAF47 Complex in Vascular Endothelial Cells is Implicated in Atherogenesis.; Authors - Nan Li ; KD Validated
human	WB	Transl Oncol; SNF5, a core subunit of SWI/SNF complex, regulates melanoma cancer cell growth, metastasis, and immune escape in response to matrix stiffness.; Authors - Ying Chen
human	WB	Bioengineered; SNF5 promotes cell proliferation and immune evasion in non-small cell lung cancer.; Authors - Ying Chen ; KD Validated