SMARCE1 Polyclonal antibody

SMARCE1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10814-1-AP

Synonyms

BAF57, BRG1 associated factor 57, SMARCE1

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Validation Data Gallery

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  WB analysis of Jurkat using 10814-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10814-1-AP (SMARCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10814-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10814-1-AP (SMARCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10814-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10814-1-AP (SMARCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10814-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10814-1-AP (SMARCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10814-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10814-1-AP (SMARCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10814-1-AP

  IP result of anti-SMARCE1 (IP:10814-1-AP, 4ug; Detection:10814-1-AP 1:800) with HEK-293 cells lysate 880ug.

• 

  IHC staining of human prostate cancer using 10814-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10814-1-AP (SMARCE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10814-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10814-1-AP (SMARCE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, HeLa cells, MCF-7 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10814-1-AP targets SMARCE1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMARCE1 fusion protein Ag1161
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC007082
• Gene Symbol: SMARCE1
• Gene ID (NCBI): 6605
• RRID: AB_2192174
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Via antagonizing chromatin-mediated transcriptional repression, the SWI/SNF complex in S. cerevisiae and Drosophila is thought to facilitate transcriptional activation of specific genes. The BRG1/brm-associated factors, or SMARCE1, complex in mammals is functionally related to SWI/SNF and consists of 9 to 12 subunits, some of which are homologous to SWI/SNF subunits. It contains a DNA-binding HMG domain and performs multiple biological functions, including DNA-, protein- and chromatin-binding, transcription coactivating activity, chromatin modification, DNA-dependent negative regulation of transcription, regulation of transcription of the RNA polymerase II promoter. In addition, SMARCE1, as an ER (estrogen receptor) subtype-selective modulator, regulates ER mediated transcription in a ligand-dependent manner and also directly binds to AR (androgen receptor) to alter its activity. The calculated molecular weight of SMARCE1 is 47 kDa, but modified SMARCE1 is about 55 kDa.

Publications

Species	Application	Title
mouse	WB	iScience; WTAP boosts lipid oxidation and induces diabetic cardiac fibrosis by enhancing AR methylation; Authors - Kai Song