BAF170 Polyclonal antibody

BAF170 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12018-1-AP

Synonyms

SMARCC2, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2, Rsc8, CRACC2, BRG1-associated factor 170

---

Validation Data Gallery

• 

  WB analysis of HeLa using 12018-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12018-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12018-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12018-1-AP

  IP result of anti-BAF170 (IP:12018-1-AP, 4ug; Detection:12018-1-AP 1:700) with HeLa cells lysate 3200ug.

• 

  IHC staining of human medulloblastoma using 12018-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12018-1-AP (SMARCC2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 12018-1-AP

  Immunofluorescent analysis of HepG2 cells, using SMARCC2 antibody 12018-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: HeLa cells, A431 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human medulloblastoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below

Product Information

12018-1-AP targets BAF170 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BAF170 fusion protein Ag2634
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2
• Calculated Molecular Weight: 1214 aa, 132 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC013045
• Gene Symbol: BAF170
• Gene ID (NCBI): 6601
• RRID: AB_2192010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAF170, also named as SWI/SNF complex 170 kDa subunit or SMARCC2, is a 1214 amino acid protein, which belongs to the SMARCC family. BAF170 is ubiquitously expressed. BAF170 is involved in transcriptional activation and repression of select genes by chromatin remodeling. BAF170 may be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. BAF170 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calcualted molecular weight of BAF170, is 133 kDa, but modified BAF170 is about 170 kDa. (PMID: 8804307)

Publications

Species	Application	Title
		Mol Cell; SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.; Authors - Shibin Hu
human	WB,IP	Aging (Albany NY); FBXO28 promotes proliferation, invasion, and metastasis of pancreatic cancer cells through regulation of SMARCC2 ubiquitination; Authors - Songbai Liu
human	WB	Biochem Biophys Res Commun; SMARCC2 silencing suppresses oncogenic activation through modulation of chromatin accessibility in breast cancer; Authors - Zhaoran Sun ; KD Validated