SMARCC1 Polyclonal antibody

SMARCC1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17722-1-AP

Synonyms

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, SRG3, Rsc8, CRACC1, BRG1-associated factor 155

---

Validation Data Gallery

• 

  WB analysis using 17722-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17722-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17722-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 17722-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 17722-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17722-1-AP (SMARCC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17722-1-AP

  IP result of anti-SMARCC1 (IP:17722-1-AP, 4ug; Detection:17722-1-AP 1:600) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human kidney using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human appendicitis using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17722-1-AP (SMARCC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 17722-1-AP (SMARCC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17722-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17722-1-AP (SMARCC1 antibody) at dilution of 1:100 (under 40x lens).

---

Tested Applications

• Positive WB detected in: K-562 cells, Jurkat cells, rat brain tissue, rat testis tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human malignant melanoma tissue, human appendicitis tissue, human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

17722-1-AP targets SMARCC1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMARCC1 fusion protein Ag12109
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1
• Calculated Molecular Weight: 1105 aa, 123 kDa
• Observed Molecular Weight: 155-160 kDa
• GenBank Accession Number: BC050564
• Gene Symbol: SMARCC1
• Gene ID (NCBI): 6599
• RRID: AB_2191987
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCC1, also named as SWI/SNF complex 155 kDa subunit or BAF155, is a 1105 amino acid protein, which belongs to the SMARCC family. SMARCC1 is expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. SMARCC1 is Involved in transcriptional activation and repression of select genes by chromatin remodeling. SMARCC1 may stimulate the ATPase activity of the catalytic subunit of the complex. SMARCC1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calculated molecular weight of TCEA1 is 123 kDa, but modified SMARCC1 is about 155-160 kDa. (PMID: 24025402)

Publications

Species	Application	Title
human	WB,IP	Cancer Discov; A role for SMARCB1 in synovial sarcomagenesis reveals that SS18-SSX induces canonical BAF destruction.; Authors - Jinxiu Li
mouse	CoIP	Nucleic Acids Res; HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.; Authors - Xu Zhu
		Mol Cell Biol; Growth Factor Independence 1B-Mediated Transcriptional Repression and Lineage Allocation Require Lysine-Specific Demethylase 1-Dependent Recruitment of the BHC Complex.; Authors - David McClellan