GLUT3 Polyklonaler Antikörper

GLUT3 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

52

Rezensionen (2)

Kat-Nr. : 20403-1-AP

Synonyme

GLUT 3, GLUT3, SLC2A3

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Galerie der Validierungsdaten

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  WB analysis using 20403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20403-1-AP (GLUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20403-1-AP (GLUT3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 20403-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 20403-1-AP (GLUT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 20403-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20403-1-AP (GLUT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20403-1-AP (GLUT3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20403-1-AP (GLUT3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Caco-2 using 20403-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using GLUT3 antibody (20403-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of Caco-2 using 20403-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using GLUT3 antibody (20403-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, C6-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, Rattenhirngewebe, U-251-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Plazenta-Gewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Caco-2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 44 publications below
• IHC: See 9 publications below
• IF: See 12 publications below

Produktinformation

20403-1-AP bindet in WB, IHC, IF, ELISA GLUT3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLUT3 fusion protein Ag14203
• Vollständiger Name: solute carrier family 2 (facilitated glucose transporter), member 3
• Berechnetes Molekulargewicht: 496 aa, 54 kDa
• Beobachtetes Molekulargewicht: 48-60 kDa
• GenBank-Zugangsnummer: BC039196
• Gene symbol: SLC2A3
• Gene ID (NCBI): 6515
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glucose transporter 3 (GLUT3), also known as solute carrier family 2, facilitated glucose transporter member 3 (SLC2A3), is a transporter protein regulating glucose transport across cell membranes and is a primary glucose transporter in neurons.

What is the molecular weight of GLUT3? Is GLUT3 post-translationally modified?

The molecular weight of GLUT3 transporter is 49-52 kDa and depends on the glycosylation pattern, which is tissue-specific (PMID: 9253355 and 9889355). GLUT3 can be N-glycosylated.

What is the subcellular localization of GLUT3?

Glucose transporters, including GLUT3, are multiple-pass integral membrane proteins. GLUT3 is present at the plasma membrane but is also a subject of recycling between the plasma membrane and endosomes. 

What molecules can be transported by GLUT3?

Although the main substrate of GLUT3 transport is glucose, it can also transport mannose, galactose, and xylose.

What is the tissue expression pattern of GLUT3?

GLUT1 and GLUT3 are important for the transport of glucose into the central nervous system. While GLUT1 transports glucose through blood vessels and into astrocytes, GLUT3 is predominantly expressed in neurons, being their main glucose transporter (PMID: 9302083), and in testis (PMID: 18577699).

Publikationen

Species	Application	Title
human	WB	Mol Cancer; CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.; Authors - Bing Yao
human	WB	Cell Death Differ; A viral interferon regulatory factor degrades RNA-binding protein hnRNP Q1 to enhance aerobic glycolysis via recruiting E3 ubiquitin ligase KLHL3 and decaying GDPD1 mRNA.; Authors - Xiaoyu Qi
human	WB	Autophagy; Newcastle disease virus degrades SIRT3 via PINK1-PRKN-dependent mitophagy to reprogram energy metabolism in infected cells.; Authors - Yabin Gong
mouse	WB	Cell Rep; 16p11.2 haploinsufficiency reduces mitochondrial biogenesis in brain endothelial cells and alters brain metabolism in adult mice; Authors - Alexandria Béland-Millar
human	WB	EMBO Rep; CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.; Authors - Xiuying Zhong
human,mouse	WB,IHC,IF	EMBO Rep; METTL14 modulates glycolysis to inhibit colorectal tumorigenesis in p53-wild-type cells; Authors - Yichao Hou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

balawant (Verified Customer) (12-25-2023)

This antibody is very specific and has no background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1200
• Cell Tissue Type: Renal Cancer cell line caki-1 and caki-2

FH

Hua (Verified Customer) (02-03-2023)

This antibody works ok. There is one strong non-specific band around 40 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2, mouse liver, and mouse brain tissues

GLUT3 Antibody Western Blot validation (1:1000 dilution) in HepG2, mouse liver, and mouse brain tissues (Cat no:20403-1-AP)

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