SIRT1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

SIRT1 Monoklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

48

CloneNo.

7F6G6

Kat-Nr. : 60303-1-Ig

Synonyme

hSIR2, hSIRT1, SIR2 like protein 1, SIR2L1, SIRT1

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Galerie der Validierungsdaten

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  WB analysis using 60303-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60303-1-Ig (SIRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Hek-293 cells using 60303-1-Ig

  WB result of SIRT1antibody (60303-1-Ig, 1:500) with si-Control and si-SIRT1 transfected HEK-293 cells.

• 

  WB analysis using 60303-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60303-1-Ig (SIRT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 60303-1-Ig

  IP Result of anti-SIRT1 (IP:60303-1-Ig, 5ug; Detection:60303-1-Ig 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of human breast cancer using 60303-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60303-1-Ig (SIRT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60303-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60303-1-Ig (SIRT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60303-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60303-1-Ig (SIRT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 60303-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60303-1-Ig (SIRT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 60303-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60303-1-Ig (SIRT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 60303-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60303-1-Ig (SIRT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 60303-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60303-1-Ig(SIRT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, LNCaP-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hodengewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 38 publications below
• IHC: See 6 publications below
• IF: See 9 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

60303-1-Ig bindet in WB, IP, IHC, IF, CoIP, ELISA SIRT1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SIRT1 fusion protein Ag17677
• Vollständiger Name: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 747 aa, 82 kDa
• Beobachtetes Molekulargewicht: 110-130 kDa
• GenBank-Zugangsnummer: BC012499
• Gene symbol: SIRT1
• Gene ID (NCBI): 23411
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SIRT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SIRT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. It can shuttles between nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.

Publikationen

Species	Application	Title
	WB,IF	J Pineal Res; Melatonin Protects Mouse Testes from Palmitic Acid-Induced Lipotoxicity by Attenuating Oxidative Stress and DNA Damage in a SIRT1-Dependent Manner.; Authors - Dejun Xu
	WB,IF	Free Radic Biol Med; Menaquinone-4 prevents medication-related osteonecrosis of the jaw through the SIRT1 signaling-mediated inhibition of cellular metabolic stresses-induced osteoblast apoptosis; Authors - Yajun Cui
	IHC	Int J Mol Sci; Exposure to Bisphenol A Caused Hepatoxicity and Intestinal Flora Disorder in Rats.; Authors - Ruijing Liu
	WB	Int J Mol Sci; COX-2/sEH Dual Inhibitor Alleviates Hepatocyte Senescence in NAFLD Mice by Restoring Autophagy through Sirt1/PI3K/AKT/mTOR.; Authors - Chen-Yu Zhang
pig	WB	J Cell Physiol; Y-box binding protein 1 influences zygotic genome activation by regulating N6-methyladenosine in porcine embryos; Authors - Wen-Jie Jiang
pig	IF	J Cell Physiol; Acetyl-CoA synthases are essential for maintaining histone acetylation under metabolic stress during zygotic genome activation in pigs.; Authors - Wenjun Zhou

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