RRM1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RRM1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

49

Kat-Nr. : 10526-1-AP

Synonyme

RR1, RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleoside-diphosphate reductase large subunit

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 10526-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10526-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10526-1-AP

  A549 cells (shcontrol and shRNA of RRM1) were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:2000. .

• 

  WB analysis of rat skeletal muscle using 10526-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10526-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10526-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 10526-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 10526-1-AP

  IP result of anti-RRM1 (IP:10526-1-AP, 5ug; Detection:10526-1-AP 1:800) with K-562 cells lysate 8000ug.

• 

  IHC staining of human breast cancer using 10526-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10526-1-AP (RRM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10526-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10526-1-AP (RRM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10526-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10526-1-AP (RRM1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 10526-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10526-1-AP (RRM1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 10526-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RRM1 antibody (10526-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of Raji using 10526-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using RRM1 antibody (10526-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HeLa using 10526-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug RRM1 Polyclonal antibody (10526-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, BxPC-3-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, U-251-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, Raji-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 33 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

10526-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA RRM1 und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Krallenfrosch (Xenopus)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RRM1 fusion protein Ag0789
• Vollständiger Name: ribonucleotide reductase M1
• Berechnetes Molekulargewicht: 90 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC006498
• Gene symbol: RRM1
• Gene ID (NCBI): 6240
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribonucleoside-diphosphate reductase large subunit (RRM1) is the main enzyme for de novo deoxyribonucleotide 

synthesis necessary for DNA synthesis during the cell cycle as well as during DNA repair.

What is the molecular weight of RRM1?

The molecular weight of RRM1 is 90 kDa. RRM1 is a part of ribonucleotide reductase (RNR), which is composed 

of two large RRM1 and two small RRM2 subunits in S phase and two large RRM1 and two alternative small p53R2 

subunits in non-dividing quiescent cells (PMID: 17416930).

What is the subcellular localization of RRM1?

RRM1 localizes to the cytoplasm, where it is responsible for dNTP production, forming a complex with RRM2. 

However, RRM1 can be recruited to DNA damage sites in the nucleus via interaction with Tip60 (PMID: 20159953).

What is the tissue expression pattern of RRM1?

RRM1 is ubiquitously expressed.

How is RRM1 expression regulated during the cell cycle and upon DNA damage?

Rrm1 gene expression levels depend on the cell cycle, and the highest mRNA levels are observed in S phase. 

However, RRM1 protein levels are constant throughout the cell cycle due to long protein half-life, contrary to 

RRM2, which is actively degraded after cell exit from S phase. During DNA damage, e.g., induced with genotoxins 

or UV light, expression of RRM1 and RRM2/p53R2 is upregulated (PMID: 1551913 and 8798592).

Publikationen

Species	Application	Title
human	WB, IF	N Engl J Med; DNA synthesis and repair genes RRM1 and ERCC1 in lung cancer.; Authors - Zhong Zheng
human	IF	Lancet Oncol; The ribonucleotide reductase large subunit (RRM1) as a predictive factor in patients with cancer.; Authors - Lars Petter Jordheim
human	WB	Clin Cancer Res; PMID: 25224279; Authors - Martin Kerr ; KD Validated
human	WB, IHC, IP	Clin Cancer Res; PMID: 26228206; Authors - Francesca Vena
human	IHC	Ann Oncol; PMID: 23038758; Authors - Vilmar A C AC
	IHC	Oncotarget; Impact of molecular profiling on overall survival of patients with advanced ovarian cancer.; Authors - Thomas J Herzog

Verwandte Produkte

RRM2 Polyclonal antibody

11661-1-AP

ERCC1 Polyclonal antibody

14586-1-AP

P53 Polyclonal antibody

10442-1-AP

ERCC2 Polyclonal antibody

10818-1-AP

TPTE Polyclonal antibody

12982-1-AP