ERCC2 Polyclonal antibody

ERCC2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 10818-1-AP

Synonyms

BTF2 p80, COFS2, CXPD, EM9, ERCC2, TFIIH 80 kDa subunit, TFIIH p80, TTD, XPD, XPDC

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Validation Data Gallery

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  WB analysis of HeLa using 10818-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10818-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10818-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10818-1-AP

  IP result of anti-ERCC2 (IP:10818-1-AP, 4ug; Detection:10818-1-AP 1:1000) with HEK-293 cells lysate 1575 ug.

• 

  IHC staining of human lymphoma using 10818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lymphoma using 10818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human lymphoma using 10818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human cervical cancer using 10818-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10818-1-AP (ERCC2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10818-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10818-1-AP (ERCC2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human lymphoma tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Product Information

10818-1-AP targets ERCC2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERCC2 fusion protein Ag1038
• Full Name: excision repair cross-complementing rodent repair deficiency, complementation group 2
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC008346
• Gene Symbol: ERCC2
• Gene ID (NCBI): 2068
• RRID: AB_2231330
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Excision-repair complementing defective in Chinese hamster 2(ERCC2), belongs to the nucleotide excision repair pathway, is a component of the TFIIH transcription complex and required for the association of the CDK-activating kinase(CAK) subcomplex with TFIIH. Since TFIIH is involved in both DNA repair and cell cycle progression, ERCC2 is implicated to play an integral role in the nucleotide excision repair pathway. Also it involves in the regulation of vitamin-D receptor activity. As a part of the mitotic spindle-associated MMXD complex, ERCC2 has a role in chromosome segregation.

Publications

Species	Application	Title
human	IHC	Am J Cancer Res; Predictive value of ERCC1, ERCC2, ERCC4, and glutathione S-Transferase Pi expression for the efficacy and safety of FOLFIRINOX in patients with unresectable pancreatic cancer.; Authors - Shun Tezuka
human	IHC	Methods; Synchronous detection of miRNAs, their targets and downstream proteins in transferred FFPE sections: applications in clinical and basic research.; Authors - Zhao Jin-yao JY
human	IHC	Cancer Sci; Very low prevalence of XPD K751Q polymorphism and its association with XPD expression and outcomes of FOLFOX-4 treatment in Asian patients with colorectal carcinoma.; Authors - Lai Jiun-I JI
human	WB	Oncotarget; PMID: 27449101; Authors - Henan Zhao
human	IHC	Biomarkers; Aberrant methylation of nucleotide excision repair genes is associated with chronic arsenic poisoning; Authors - Aihua Zhang
human	IHC	J Transl Med; Proteomic analysis predicts anti-angiogenic resistance in recurred glioblastoma; Authors - Hanwool Jeon

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zizhao (Verified Customer) (08-15-2018)

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1: 1000
• Cell Tissue Type: human keratinocytes