ERCC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ERCC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 14586-1-AP

Synonyms

UV20, RAD10, DNA excision repair protein ERCC-1, COFS4

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Validation Data Gallery

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  WB analysis using 14586-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14586-1-AP (ERCC1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14586-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14586-1-AP (ERCC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 14586-1-AP

  IP result of anti-ERCC1 (IP:14586-1-AP, 3ug; Detection:14586-1-AP 1:300) with MCF-7 cells lysate 1600ug.

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  IHC staining of human testis using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 14586-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human kidney tissue, MCF-7 cells, SKOV-3 cells, T-47D cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human testis tissue, human brain tissue, human ovary tissue, human skin tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 16 publications below
• IHC: See 4 publications below

Product Information

14586-1-AP targets ERCC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERCC1 fusion protein Ag6112
• Full Name: excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC052813
• Gene Symbol: ERCC1
• Gene ID (NCBI): 2067
• RRID: AB_2100027
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Excision Repair Cross Complementing 1 (ERCC1) is a structure-specific endonuclease that is responsible for the 5'-incision during DNA repair. It forms a complex with ERCC11, XPF and ERCC4, which are required in both recombinatorial repair and nucleotide excision repair. It has been found that ERCC1, together with RRM1, are determinants of survival after surgical treatment of early-stage, non-small-cell lung cancer. (Ref: Simon, ER. 2005). The calculated molecular weight of ERCC1 is 33 kDa, but the modified ERCC1 is about38 kDa.

Publications

Species	Application	Title
human	WB	Blood Cancer J; Severe ineffective erythropoiesis discriminates prognosis in myelodysplastic syndromes: analysis based on 776 patients from a single centre.; Authors - Huijun Huang ; KD Validated
human	WB	Cell Death Dis; PFKFB3 blockade inhibits hepatocellular carcinoma growth by impairing DNA repair through AKT.; Authors - Wen-Kai Shi
human	WB	J Cell Mol Med; Berberine attenuates XRCC1-mediated base excision repair and sensitizes breast cancer cells to the chemotherapeutic drugs.; Authors - Xingjie Gao
human	WB	Oncotarget; Functional characterization of a novel transcript of ERCC1 in chemotherapy resistance of ovarian cancer.; Authors - Jia Liu
human	WB, IHC	Oncol Res; The AKT/GSK3-Mediated Slug Expression Contributes to Oxaliplatin Resistance in Colorectal Cancer via Upregulation of ERCC1.; Authors - Wei Wei ; KD Validated
human	WB	Cancer Cell Int; Mechanistic study of lncRNA UCA1 promoting growth and cisplatin resistance in lung adenocarcinoma.; Authors - Jiali Fu