RB1 Polyklonaler Antikörper

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• KD/KO Validated

RB1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Kat-Nr. : 17218-1-AP

Synonyme

OSRC, p105 Rb, pp110, pRb, Rb, RB1, retinoblastoma 1

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Galerie der Validierungsdaten

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  WB analysis of HT-1080 using 17218-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 17218-1-AP (RB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 17218-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 17218-1-AP (RB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 22 publications below

Produktinformation

17218-1-AP bindet in WB, ELISA RB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RB1 fusion protein Ag11035
• Vollständiger Name: retinoblastoma 1
• Berechnetes Molekulargewicht: 928 aa, 106 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC039060
• Gene symbol: RB1
• Gene ID (NCBI): 5925
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets.; Authors - Jun Lu
	WB	J Hazard Mater; Neurotoxicity of aluminum oxide nanoparticles and their mechanistic role in dopaminergic neuron injury involving p53-related pathways.; Authors - Huanliang Liu
human	WB	Aging Cell; Berberine ameliorates cellular senescence and extends the lifespan of mice via regulating p16 and cyclin protein expression.; Authors - Yao Dang
zebrafish	WB	Development; Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1.; Authors - Zili Zhang ; KO Validated
human	WB	Oncotarget; High expression of N-myc (and STAT) interactor predicts poor prognosis and promotes tumor growth in human glioblastoma.; Authors - Delong Meng
human	WB	Oncotarget; Drp1-mediated mitochondrial fission promotes cell proliferation through crosstalk of p53 and NF-κB pathways in hepatocellular carcinoma.; Authors - Lei Zhan

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