RAB27B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB27B Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

40

Rezensionen (1)

Kat-Nr. : 13412-1-AP

Synonyme

C25KG, Ras-related protein Rab-27B, Ras related protein Rab 27B, EC:3.6.5.2

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Galerie der Validierungsdaten

• 

  WB analysis of rat spleen using 13412-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 13412-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13412-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 13412-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13412-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human oesophagus cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human prostate cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 13412-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13412-1-AP (RAB27B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of U-87 MG using 13412-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using RAB27B antibody (13412-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenmilzgewebe, A375-Zellen, DU 145-Zellen, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Hodengewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Ösophaguskarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U-87 MG-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 11 publications below
• WB: See 33 publications below
• IHC: See 13 publications below
• IF: See 7 publications below

Produktinformation

13412-1-AP bindet in WB, IHC, IF/ICC, ELISA RAB27B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB27B fusion protein Ag4064
• Vollständiger Name: RAB27B, member RAS oncogene family
• Berechnetes Molekulargewicht: 218 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC027474
• Gene symbol: RAB27B
• Gene ID (NCBI): 5874
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.

Publikationen

Species	Application	Title
mouse	IHC	Nature; Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth.; Authors - Lin Zhang
human	WB, IHC	Cell; Exosome transfer from stromal to breast cancer cells regulates therapy resistance pathways.; Authors - Mirjam C Boelens
human	WB	Immunity; MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation.; Authors - Seongmin Yoon
human	WB	Adv Sci (Weinh); Exosome Release Is Regulated by mTORC1.; Authors - Wenchong Zou
mouse	IF	Nat Commun; Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.; Authors - I-Ling Lu
human	WB	Nat Commun; Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.; Authors - Akiko Takahashi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kacie (Verified Customer) (09-12-2022)

Antibody works well for both ICC and WB.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: M17 Cells

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