MLPH Polyclonal antibody

• Featured Product
• KD/KO Validated

MLPH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 10338-1-AP

Synonyms

SlaC2-a, SLAC2A, SLAC2 A, Melanophilin, Exophilin-3

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Validation Data Gallery

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  WB analysis of A375 using 10338-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10338-1-AP (Melanophilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 10338-1-AP

  IP result of anti-Melanophilin (IP:10338-1-AP, 4ug; Detection:10338-1-AP 1:600) with A375 cells lysate 3600ug.

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  IHC staining of human skin cancer using 10338-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10338-1-AP (Melanophilin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 10338-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10338-1-AP (Melanophilin antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 10338-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10338-1-AP (Melanophilin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 10338-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Melanophilin antibody (10338-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A375 cells
• Positive IP detected in: A375 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 19 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

10338-1-AP targets MLPH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MLPH fusion protein Ag0316
• Full Name: melanophilin
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66 kDa, 80 kDa
• GenBank Accession Number: BC001653
• Gene Symbol: MLPH
• Gene ID (NCBI): 79083
• RRID: AB_2146104
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.

Publications

Species	Application	Title
human	WB, IP	J Allergy Clin Immunol; Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding.; Authors - Valentina Cetica
human	WB	Theranostics; A novel function of Prohibitin on melanosome transport in melanocytes.; Authors - Chan Song Jo ; KD Validated
human	WB,IF,IP	Oncogene; O-GlcNAcylation of melanophilin enhances radiation resistance in glioblastoma via suppressing TRIM21 mediated ubiquitination; Authors - Lei Xu
mouse	WB	Elife; Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis; Authors - Kunli Zhao
mouse	WB,IF	Part Fibre Toxicol; Coal dust exposure triggers heterogeneity of transcriptional profiles in mouse pneumoconiosis and Vitamin D remedies.; Authors - Min Mu
human	WB	J Invest Dermatol; Knockdown of myosin Va isoforms by RNAi as a tool to block melanosome transport in primary human melanocytes.; Authors - Van Gele Mireille M

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