UNC13D/Munc13-4 Polyclonal antibody

UNC13D/Munc13-4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 16905-1-AP

Synonyms

FHL3, HLH3, HPLH3, Munc13 4, Munc13-4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D, UNC13D/Munc13-4

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Validation Data Gallery

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  WB analysis using 16905-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16905-1-AP (UNC13D/Munc13-4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 16905-1-AP

  WB result of UNC13D/Munc13-4 antibody (16905-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-UNC13D/Munc13-4 transfected K-562 cells.

• 

  WB analysis using 16905-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16905-1-AP (UNC13D/Munc13-4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 16905-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16905-1-AP (UNC13D/Munc13-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16905-1-AP

  IP result of anti-UNC13D/Munc13-4 (IP:16905-1-AP, 5ug; Detection:16905-1-AP 1:300) with Jurkat cells lysate 2400ug.

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  IHC staining of human thymus using 16905-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 16905-1-AP (UNC13D/Munc13-4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thymus using 16905-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 16905-1-AP (UNC13D/Munc13-4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, human placenta tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human thymus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below

Product Information

16905-1-AP targets UNC13D/Munc13-4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UNC13D/Munc13-4 fusion protein Ag10519
• Full Name: unc-13 homolog D (C. elegans)
• Calculated Molecular Weight: 1090 aa, 103 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC067084
• Gene Symbol: UNC13D
• Gene ID (NCBI): 201294
• RRID: AB_2212713
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells; Authors - Tatsuaki Kurosaki
human	WB	J Exp Med; Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.; Authors - Frank Cichocki
human	WB	Blood; Familial hemophagocytic lymphohistiocytosis type 3 (FHL3) caused by deep intronic mutation and inversion in UNC13D.; Authors - Meeths Marie M
human	WB	Front Immunol; Alternative UNC13D Promoter Encodes a Functional Munc13-4 Isoform Predominantly Expressed in Lymphocytes and Platelets.; Authors - Donatella Galgano
human	WB,IF	Mol Biol Cell; A novel Munc13-4/S100A10/Annexin A2 complex promotes Weibel-Palade body exocytosis in endothelial cells.; Authors - Tarek Chehab