RAB10 Polyklonaler Antikörper

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• KD/KO Validated

RAB10 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Kat-Nr. : 11808-1-AP

Synonyme

Ras-related protein Rab-10, Ras related protein Rab 10, EC:3.6.5.2

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Galerie der Validierungsdaten

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  WB analysis using 11808-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11808-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11808-1-AP

  WB result of RAB10 antibody (11808-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB10 transfected HeLa cells.

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  WB analysis of NIH/3T3 using 11808-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11808-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 11808-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 11808-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11808-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11808-1-AP (RAB10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11808-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11808-1-AP (RAB10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 11808-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RAB10 antibody (11808-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, C6-Zellen, HeLa-Zellen, MCF-7-Zellen, MDCK-Zellen, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Produktinformation

11808-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA RAB10 und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB10 fusion protein Ag2473
• Vollständiger Name: RAB10, member RAS oncogene family
• Berechnetes Molekulargewicht: 200 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC000896
• Gene symbol: RAB10
• Gene ID (NCBI): 10890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB10 belongs to the RAS superfamily of small GTPases. RAB10 localizes to a system of tubules and vesicles, and it may be involved in vesicular trafficking and neurotransmitter release. It is also associated with Glut4 translocation in adipocytes.

Publikationen

Species	Application	Title
rat	WB,IP,IF	Cell Res; MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development.; Authors - Xiao-Hui Xu
rat, zebrafish	WB,IF	Nat Commun; Myosin Vb controls biogenesis of post-Golgi Rab10 carriers during axon development.; Authors - Liu Yang Y
mouse	IF	Proc Natl Acad Sci U S A; Ras-related protein Rab10 facilitates TLR4 signaling by promoting replenishment of TLR4 onto the plasma membrane.; Authors - Wang Di D
rat	WB	Sci Signal; Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections.; Authors - Kong-Yan Wu
rat	WB	J Neurosci; Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.; Authors - Pavlos Nathan J NJ
rat	WB,IF	J Neurosci; JIP1 Mediates Anterograde Transport of Rab10 Cargos during Neuronal Polarization.; Authors - Cai-Yun Deng

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