Paxillin Polyklonaler Antikörper

Paxillin Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Rezensionen (2)

Kat-Nr. : 10029-1-Ig

Synonyme

PXN

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 10029-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10029-1-Ig (PXN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10029-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10029-1-Ig (Paxillin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10029-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10029-1-Ig (PXN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10029-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10029-1-Ig (PXN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10029-1-Ig

  IP result of anti-Paxillin (IP:10029-1-Ig, 3ug; Detection:10029-1-Ig 1:1000) with mouse brain tissue lysate 7500ug.

• 

  IHC staining of human liver cancer using 10029-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10029-1-Ig(PXN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 10029-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10029-1-Ig(PXN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 10029-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10029-1-Ig (Paxillin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 10029-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using Paxillin antibody (10029-1-Ig) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue).

• 

  IF Staining of HepG2 using 10029-1-Ig

  Immunofluorescent analysis of -20℃ Ethanol fixed HepG2 cells using 10029-1-Ig (Paxillin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L). The cytoskeleton was labelled in red with 66031-1-Ig (alpha tubulin). DAPI (blue).

• 

  IF Staining of HepG2 using 10029-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10029-1-Ig (Paxillin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NIH/3T3 using 10029-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed NIH/3T3 cells using 10029-1-Ig(Paxillin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, COLO 320-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HUVEC-Zellen, HepG2-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 7 publications below

Produktinformation

10029-1-Ig bindet in WB, IHC, IF/ICC, IP, ELISA Paxillin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: paxillin
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: NM_002859
• Gene symbol: Paxillin
• Gene ID (NCBI): 5829
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PXN (paxillin) is a 68 kDa scaffold protein that interacts with multiple structural and signaling proteins and regulates cell adhesion, migration, proliferation, and apoptosis. PXN is thought to play an important role in tumor migration, invasion, and metastasis (21045234). PXN has been identified as a direct substrate of protein tyrosine phosphatase receptor-type T (PTPRT), a potent tumor suppressor gene. Increased phospho-PXN at tyrosine residue 88 (Y88) has been found as a common feature of human colon cancers (20133777).

Publikationen

Species	Application	Title
human	WB	Mol Cell; Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine.; Authors - Daniel Arango
rat	IHC	Sci Adv; Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction; Authors - Yuzhi Sun
human	IF	Bone Res; Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma; Authors - Ming Luo
human	IF	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
human	WB	Cell Death Dis; ZC3H15 promotes glioblastoma progression through regulating EGFR stability.; Authors - Jianbing Hou
human	IF	Acta Pharmacol Sin; Reduced intracellular chloride concentration impairs angiogenesis by inhibiting oxidative stress-mediated VEGFR2 activation.; Authors - Kai Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Thomas (Verified Customer) (09-22-2022)

Works in WB (pulldown via PAK1) and IF (costained with F-Actin in cyan)

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:100 (IF), 1:500 (WB)
• Cell Tissue Type: Human Brain Microvascular endothelial cell line

Paxillin Antibody Western Blot,Immunofluorescence validation (1:100 (IF), 1:500 (WB) dilution) in Human Brain Microvascular endothelial cell line (Cat no:10029-1-Ig)

FH

Boyan (Verified Customer) (03-11-2019)

For WB, besides the expected band, it also recognised some other non-specific bands; for IF, it could label the cell cortex localisation, partially co-localised with Actin.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF

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