Alpha Parvin/Actopaxin Polyclonal antibody

• Featured Product
• KD/KO Validated

Alpha Parvin/Actopaxin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11202-1-AP

Synonyms

Actopaxin, Alpha parvin, Alpha Parvin/Actopaxin, CH ILKBP, MXRA2, PARVA, parvin, alpha, α-Parvin

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Validation Data Gallery

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  WB analysis of HeLa using 11202-1-AP

  WB result of α-Parvin/Actopaxin antibody (11202-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-α-Parvin/Actopaxin transfected HeLa cells.

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  WB analysis of human liver using 11202-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11202-1-AP (Actopaxin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11202-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11202-1-AP (Actopaxin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11202-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11202-1-AP (Actopaxin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 11202-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11202-1-AP (Actopaxin antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: human liver tissue, Jurkat cells, HeLa cells, human kidney tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

11202-1-AP targets Alpha Parvin/Actopaxin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha Parvin/Actopaxin fusion protein Ag1694
• Full Name: parvin, alpha
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: BC016713
• Gene Symbol: Alpha Parvin/PARVA
• Gene ID (NCBI): 55742
• RRID: AB_2236617
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Transl Androl Urol; Downregulation of PARVA promotes metastasis by modulating integrin-linked kinase activity and regulating MAPK/ERK and MLC2 signaling in prostate cancer.; Authors - Cong Huang
mouse	WB	Cell Death Differ; Integrin-KCNB1 potassium channel complexes regulate neocortical neuronal development and are implicated in epilepsy; Authors - Alessandro Bortolami
human	IHC	Cells; α-Parvin Expression in Breast Cancer Tissues: Correlation with Clinical Parameters and Prognostic Significance; Authors - Midori Takeda