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PR repeat Polyklonaler Antikörper

PR repeat Polyklonal Antikörper für ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF, Dot Blot, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23979-1-AP

Synonyme

C9RAN, GGGCC, poly PR, poly-PR, PR repeat, PRPRPRPRP

Galerie der Validierungsdaten

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  • ELISA experiment of peptide using PR repeat Polyclonal antibody (23979-1-AP)

    ELISA experiment of peptide using 23979-1-AP

    PR repeat Antibody (23979-1-AP) tested in ELISA. PR repeat peptide was coated onto the microplates at 1.5 µg/well and then incubated with dilution series of PR repeat antibody (23979-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

Geprüfte Anwendungen

Erfolgreiche Detektion in ELISApeptide

Empfohlene Verdünnung

AnwendungVerdünnung
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

23979-1-AP bindet in WB, IHC, IF, Dot Blot, ELISA PR repeat und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, drosophila, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name PR repeat
Gene symbol
Gene ID (NCBI)
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446).This antibody is used to detect the "PR repeat" sequence. This antibody detects the PRPRPRPRP peptide with dilution 1:4,500 in Elisa.

Publikationen

SpeciesApplicationTitle
humanIHC

Nat Neurosci

RPS25 is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats.

Authors - Shizuka B Yamada
View Article
ratWB, IF

Neuron

Antisense Proline-Arginine RAN Dipeptides Linked to C9ORF72-ALS/FTD Form Toxic Nuclear Aggregates that Initiate In Vitro and In Vivo Neuronal Death.

Authors - Xinmei Wen
View Article
mouseIF

Nat Commun

Motor dysfunction and neurodegeneration in a C9orf72 mouse line expressing poly-PR.

Authors - Zongbing Hao
View Article
humanWB, IHC

Acta Neuropathol

Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers.

Authors - Ian R A Mackenzie
View Article
humanWB

EMBO J

NDST3 deacetylates α-tubulin and suppresses V-ATPase assembly and lysosomal acidification.

Authors - Qing Tang
View Article
humanWB

EMBO J

The RNA exosome complex degrades expanded hexanucleotide repeat RNA in C9orf72 FTLD/ALS.

Authors - Yuya Kawabe
View Article