PPAR Gamma Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PPAR Gamma Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

395

Rezensionen (3)

Kat-Nr. : 16643-1-AP

Synonyme

CIMT1, NR1C3, PPAR gamma, PPARG, PPARG1, PPARG2, PPARgamma, PPARγ

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Galerie der Validierungsdaten

• 

  WB analysis using 16643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16643-1-AP (PPAR Gamma antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16643-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16643-1-AP (PPAR Gamma antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16643-1-AP (PPAR Gamma antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16643-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16643-1-AP (PPAR gamma antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16643-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16643-1-AP (PPAR gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 16643-1-AP

  IP result of anti-PPAR Gamma(IP:16643-1-AP, 4ug; Detection:16643-1-AP 1:2000) with HL-60 cells lysate 2440 ug.

• 

  IHC staining of human prostate cancer using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat liver using 16643-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using PPAR Gamma antibody (16643-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat liver using 16643-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using PPAR Gamma antibody (16643-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 16643-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PPAR Gamma (16643-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HL-60-Zellen, humanes Herzgewebe, Mausherzgewebe, MCF-7-Zellen, U-937-Zellen
• Erfolgreiche IP: HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Plazenta-Gewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Rattenlebergewebe
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 16 publications below
• WB: See 364 publications below
• IHC: See 38 publications below
• IF: See 34 publications below
• IP: See 7 publications below
• CoIP: See 6 publications below
• ChIP: See 7 publications below

Produktinformation

16643-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA PPAR Gamma und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ente, hamster, Hausschwein, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPAR Gamma fusion protein Ag10005
• Vollständiger Name: peroxisome proliferator-activated receptor gamma
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC006811
• Gene symbol: PPARG
• Gene ID (NCBI): 5468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated intracellular transcription factors, members of the nuclear hormone receptor superfamily (NR), that includes estrogen, thyroid hormone receptors, retinoic acid, Vitamin D3 as well as retinoid X receptors (RXRs). The PPAR subfamily consists of three subtypes encoded by distinct genes denoted PPARα (NR1C1), PPARβ/δ (NR1C2) and PPARγ (NR1C3), which are activated by selective ligands. PPARγ, also named as PPARG, contains one nuclear receptor DNA-binding domain and is a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. It plays an important role in the regulation of lipid homeostasis, adipogenesis, ins resistance, and development of various organs. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) and may be associated with susceptibility to obesity. Defects in PPARG can lead to type 2 ins-resistant diabetes and hypertension. PPARG mutations may be associated with colon cancer. Genetic variations in PPARG are associated with susceptibility to glioma type 1 (GLM1). PPARG has two isoforms with molecular weight 57 kDa and 54 kDa (PMID: 9831621), but modified PPARG is about 67 KDa (PMID: 16809887). PPARG2 is a splice variant and has an additional 30 amino acids at the N-terminus (PMID: 15689403). Experimental data indicate that a 45 kDa protein displaying three different sequences immunologically related to the nuclear receptor PPARG2 is located in mitochondria (mt-PPAR). However, the molecular weight of this protein is clearly less when compared to that of PPARG2 (57 kDa) (PMID: 10922459). PPARG has been reported to be localized mainly (but not always) in the nucleus. PPARG can also be detected in the cytoplasm and was reported to possess extra-nuclear/non-genomic actions (PMID: 17611413; 19432669; 14681322).

Publikationen

Species	Application	Title
mouse	WB	Nat Nanotechnol; Photoacoustic molecular imaging-escorted adipose photodynamic-browning synergy for fighting obesity with virus-like complexes.; Authors - Ronghe Chen
mouse	WB,IF	ACS Nano; Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy.; Authors - Mengshi Jiang
mouse	WB	J Pineal Res; Single-cell RNA sequencing of preadipocytes reveals the cell fate heterogeneity induced by melatonin.; Authors - Zhenhui Li
mouse	WB, IHC	Nat Commun; N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.; Authors - Yanying Wang
mouse	WB	Nat Commun; METTL3 is essential for postnatal development of brown adipose tissue and energy expenditure in mice.; Authors - Yuqin Wang
mouse	WB	Research (Wash D C); Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis; Authors - Lang Linghu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Surendra (Verified Customer) (12-30-2023)

I purchased this antibody by seeing single band on web page, but it is giving several bands, so its really tough to determine which band is real PPAR gamma.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Pancreatic Cancer Cells

FH

Giulia (Verified Customer) (02-21-2022)

The antibody performed well

• Applications: Immunofluorescence

FH

Boyan (Verified Customer) (04-24-2019)

By WB, this antibody labeled many bands, which makes it very hard to distinguish which one is the actual PPARg.

• Applications: Western Blot,
• Cell Tissue Type: 3T3 L1

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