PGRMC1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PGRMC1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 12990-1-AP

Synonyme

PGRMC, mPR, Membrane-associated progesterone receptor component 1, HPR6.6

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 12990-1-AP

  WB result of PGRMC1 antibody (12990-1-AP; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PGRMC1 transfected HEK-293 cells.

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  WB analysis using 12990-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12990-1-AP (PGRMC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12990-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12990-1-AP (PGRMC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12990-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12990-1-AP (PGRMC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 12990-1-AP

  IP result of anti-PGRMC1 (IP:12990-1-AP, 3ug; Detection:12990-1-AP 1:1000) with MCF-7 cells lysate 1600ug.

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  IHC staining of human cervical cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12990-1-AP (PGRMC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12990-1-AP (PGRMC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12990-1-AP (PGRMC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12990-1-AP (PGRMC1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12990-1-AP (PGRMC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12990-1-AP (PGRMC1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 12990-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PGRMC1 antibody (12990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, Maus-Eierstockgewebe, Mauslebergewebe, Mausnierengewebe, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Ovarialkarzinomgewebe, Maus-Eierstockgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

12990-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PGRMC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PGRMC1 fusion protein Ag3643
• Vollständiger Name: progesterone receptor membrane component 1
• Berechnetes Molekulargewicht: 195 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22-28 kDa
• GenBank-Zugangsnummer: BC034238
• Gene symbol: PGRMC1
• Gene ID (NCBI): 10857
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Progesterone receptor membrane component 1 (PGRMC1) is a member of a multi-protein progesterone-binding complex. However, PGRMC1 shares homology with cytochrome b5-related proteins rather than hormone receptors (PMID: 18992768). It is a heme binding protein with biding sites for Src homology (SH2) and SH3 domain-containing proteins (PMID: 17583495). PGRMC1 is overexpressed in a variety of cancers, and thus represents an important biomarker for cancer progression and a potential target for anticancer drugs (PMID: 21730960). In nonmalignant tissues, PGRMC1 is highly expressed in the liver and kidney (PMID: 9705155; 20164297).

Publikationen

Species	Application	Title
human	WB	EBioMedicine; The Sigma-2 Receptor and Progesterone Receptor Membrane Component 1 are Different Binding Sites Derived From Independent Genes.; Authors - Uyen B Chu ; KO Validated
human	IHC	Int J Cancer; Elevated progesterone receptor membrane component 1/sigma-2 receptor levels in lung tumors and plasma from lung cancer patients.; Authors - Mir Shakeel U R SU
rat	WB	Transl Psychiatry; A potential mechanism underlying atypical antipsychotics-induced lipid disturbances.; Authors - H L Cai
rat	WB	Front Pharmacol; A Potential Mechanism Underlying the Therapeutic Effects of Progesterone and Allopregnanolone on Ketamine-Induced Cognitive Deficits.; Authors - Ting Cao
human	WB	Biol Reprod; Hyaluronic Acid Promotes the Expression of Progesterone Receptor Membrane Component 1 via Epigenetic Silencing of miR-139-5p in Granulosa Cells.; Authors - Guangfeng Zhao
rat	WB	J Cell Physiol; Progesterone maintains the status of granulosa cells and slows follicle development partly through PGRMC1.; Authors - Xiao-Hua Yuan

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