PDI Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PDI Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte, Zebrafisch und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

59

Rezensionen (2)

Kat-Nr. : 11245-1-AP

Synonyme

P4HB, GIT, ERBA2L, EC:5.3.4.1, DSI

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Galerie der Validierungsdaten

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  WB analysis using 11245-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11245-1-AP (PDI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11245-1-AP

  WB result of PDI antibody (11245-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDI transfected HEK-293 cells.

• 

  IP experiment of HepG2 using 11245-1-AP

  IP result of anti-PDI (IP:11245-1-AP, 4ug; Detection:11245-1-AP 1:2000) with HepG2 cells lysate 2600 ug.

• 

  IHC staining of human prostate cancer using 11245-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11245-1-AP (PDI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11245-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PDI antibody (11245-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue).

• 

  FC experiment of HeLa using 11245-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PDI (11245-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HEK-293-Zellen, HepG2-Zellen, Mausherzgewebe, Rattenhirngewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 35 publications below
• IHC: See 11 publications below
• IF: See 19 publications below
• FC: See 1 publications below

Produktinformation

11245-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA PDI und zeigt Reaktivität mit human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: human, Maus, Ratte, Zebrafisch
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDI fusion protein Ag1747
• Vollständiger Name: prolyl 4-hydroxylase, beta polypeptide
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC014504
• Gene symbol: PDI
• Gene ID (NCBI): 5034
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

Publikationen

Species	Application	Title
human	WB	Nature; 7-Dehydrocholesterol dictates ferroptosis sensitivity; Authors - Yaxu Li
human,mouse	IHC	Nature; Heart repair by reprogramming non-myocytes with cardiac transcription factors.; Authors - Song Kunhua K
human	IF	J Thromb Haemost; VWF-Gly2752Ser, a novel non-cysteine substitution variant in the CK domain, exhibits severe secretory impairment by hampering C-terminal dimer formation.; Authors - Shuichi Okamoto
	IF	Mol Cell; The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.; Authors - Linyu Sun
human	IHC, WB	J Extracell Vesicles; Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.; Authors - Xiaohan Gao
mouse	IF	Sci Adv; Role of Ca2+ transients at the node of the mouse embryo in breaking of left-right symmetry.; Authors - Katsutoshi Mizuno

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-19-2024)

Worked well at 1:2000

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: MSCs

PDI Antibody Western Blot validation (1:2000 dilution) in MSCs (Cat no:11245-1-AP)

FH

Anastasia (Verified Customer) (08-07-2024)

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Zebrafish embryos

PDI Antibody Western Blot validation (1:500 dilution) in Zebrafish embryos (Cat no:11245-1-AP)

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C

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18233-1-AP

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23185-1-AP

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12148-1-AP