OCT4 Monoklonaler Antikörper

OCT4 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

31

CloneNo.

1C4B6

Kat-Nr. : 60242-1-Ig

Synonyme

Oct 3, Oct 4, OCT3, OCT3/4, OCT4, Octamer binding protein 3, Octamer binding protein 4, OTF 3, OTF3, OTF4, POU class 5 homeobox 1, POU5F1

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Galerie der Validierungsdaten

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  WB analysis using 60242-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60242-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 60242-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 60242-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60242-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60242-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 60242-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60242-1-Ig (OCT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 60242-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60242-1-Ig (OCT4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60242-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60242-1-Ig (OCT4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60242-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60242-1-Ig (OCT4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 60242-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60242-1-Ig (OCT4 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Hirngewebe, L02-Zellen, NCCIT-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 27 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Produktinformation

60242-1-Ig bindet in WB, IHC, IF, ELISA OCT4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: OCT4 fusion protein Ag1794
• Vollständiger Name: POU class 5 homeobox 1
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 50-52 kDa
• GenBank-Zugangsnummer: BC020712
• Gene symbol: POU5F1
• Gene ID (NCBI): 5460
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Background

Octamer-binding protein 4 (OCT4), also known as POU5F1 or OCT3/4, is a member of the POU gene family and encoded by the human gene POU5F1 (POU domain class 5 transcription factor 1). OCT4 is a member of the Octamer class of transcription factors that recognize the 8-base pair consensus motif 5'-ATGCAAAT-3'. Expression of OCT4 is associated with an undifferentiated, pluripotent stem cell phenotype and tumors. OCT4 is also expressed in the developing brain, with the highest levels found in the cortex, olfactory bulb, and the hippocampus.

 What is the molecular weight of OCT4?

The molecular weight of OCT4 is 38 kDa.

 What are the isoforms of OCT4?

The human POU51F gene consists of five exons located on chromosome 6 and can generate three mRNA isoforms through alternative splicing - OCT4A, OCT4B, and OCT4B1 (PMID: 18787205). OCT4A and OCT4B1 orchestrate gene transcription in the nucleus supporting self-renewal and pluripotency maintenance in ESCs and embryonal carcinoma cells, whereas OCT4B is localized in the cytoplasm in various non-pluripotent cell types and cannot sustain self-renewal and pluripotency.

 What is OCT4's involvement in pluripotency and self-renewal?

OCT4 forms a trimeric complex with SOX2 and DNA to control the expression of genes involved with embryonic development, such as FGF4 (PMID: 10801796), UTF1 (PMID: 10409735), or even NANOG (PMID: 15743839). OCT4 is critical for early embryogenesis (PMID: 9814708) and for embryonic stem cell pluripotency (PMID: 16153702). High levels of OCT4 are associated with the ability to self-renew, which is emphasized by OCT4 gene knockdowns promoting differentiation (PMID: 10742100). OCT4 is also one of the four key transcription factors used to generate induced pluripotent stem cells (iPSCs) by reprogramming fibroblasts to a pluripotent state (PMID: 16904174).

Publikationen

Species	Application	Title
human	WB,IHC	EBioMedicine; PMID: 31594754; Authors - Yiyi Liu
human	WB	J Hematol Oncol; A novel lncRNA ROPM-mediated lipid metabolism governs breast cancer stem cell properties.; Authors - Shuiqing Liu
human	WB	Theranostics; Sulforaphane Inhibits the Acquisition of Tobacco Smoke-Induced Lung Cancer Stem Cell-Like Properties via the IL-6/ΔNp63α/Notch Axis.; Authors - Chunfeng Xie
human	WB	Cancer Commun (Lond); KDELR2 promotes breast cancer proliferation via HDAC3-mediated cell cycle progression.; Authors - Haoran Wei
human	WB	Front Oncol; PIWIL1 Drives Chemoresistance in Multiple Myeloma by Modulating Mitophagy and the Myeloma Stem Cell Population.; Authors - Yajun Wang
human	IF	Stem Cell Res; Generation of a human induced pluripotent stem cell line (INNDSUi003-A) derived from patient with Becker muscular dystrophy (BMD).; Authors - Xinbo Ji

Verwandte Produkte

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14295-1-AP

SOX2 Polyclonal antibody

11064-1-AP

EWS Polyclonal antibody

55191-1-AP

KLF4 Polyclonal antibody

11880-1-AP

OCT1 Polyclonal antibody

10387-1-AP

Lin28A-specific Polyclonal antibody

16177-1-AP