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TrkC Monoklonaler Antikörper

TrkC Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A7F11

Kat-Nr. : 66380-1-Ig

Synonyme

GP145 TrkC, gp145(trkC), NT 3 growth factor receptor, NTRK3, Trk C, TRKC, TrkC tyrosine kinase

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of various lysates using TrkC Monoclonal antibody (66380-1-Ig)

    WB analysis using 66380-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66380-1-Ig (TrkC antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using TrkC Monoclonal antibody (66380-1-Ig)

    WB analysis of fetal human brain using 66380-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66380-1-Ig (TrkC antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using TrkC Monoclonal antibody (66380-1-Ig)

    WB analysis of SH-SY5Y using 66380-1-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66380-1-Ig (TrkC antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using TrkC Monoclonal antibody (66380-1-Ig)

    WB analysis of PC-12 using 66380-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 66380-1-Ig (TrkC antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using TrkC Monoclonal antibody (66380-1-Ig)

    FC experiment of SH-SY5Y using 66380-1-Ig

    1X10^6 SH-SY5Y cells were stained with 0.20 ug Anti-Human TrkC (66380-1-Ig, Clone:2A7F11) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Cells were fixed with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHausschwein-Hirngewebe, fetales humanes Hirngewebe, Maushirngewebe, PC-12-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen
Erfolgreiche Detektion in FCSH-SY5Y-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Durchflusszytometrie (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66380-1-Ig bindet in WB, FC, ELISA TrkC und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen TrkC fusion protein Ag2635
Vollständiger Name neurotrophic tyrosine kinase, receptor, type 3
Berechnetes Molekulargewicht 839 aa, 94 kDa
Beobachtetes Molekulargewicht 110 kDa, 160 kDa
GenBank-ZugangsnummerBC013693
Gene symbol NTRK3
Gene ID (NCBI) 4916
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
WB protocol for TrkC antibody 66380-1-IgProtokoll herunterladen
FC protocol for TrkC antibody 66380-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen