TrkB Polyclonal antibody

TrkB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, Peptide array

Conjugate

Unconjugated

Publications

62

Cat no : 13129-1-AP

Synonyms

NTRK2, Tkrb, NTRK 2, GP145-TrkB, GP145 TrkB

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Validation Data Gallery

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  WB analysis using 13129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 13129-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 13129-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spinal cord using 13129-1-AP

  mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 13129-1-AP (TrkB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse cerebellum using 13129-1-AP

  IP result of anti-TrkB (IP:13129-1-AP, 4ug; Detection:13129-1-AP 1:300) with mouse cerebellum tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 13129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13129-1-AP (TrkB antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13129-1-AP (TrkB antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 13129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13129-1-AP (TrkB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 13129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13129-1-AP (TrkB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 13129-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 13129-1-AP (TrkB antibody) at dilution of 1:25 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SH-SY5Y using 13129-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 13129-1-AP (TrkB antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human cerebellum tissue, human stomach tissue, mouse spinal cord tissue, pig cerebellum tissue, rat brain tissue, mouse cerebellum tissue
• Positive IP detected in: mouse cerebellum tissue
• Positive IHC detected in: mouse brain tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 57 publications below
• IHC: See 5 publications below
• IF: See 8 publications below
• CoIP: See 1 publications below

Product Information

13129-1-AP targets TrkB in WB, IHC, IF/ICC, IP, CoIP, ELISA, Peptide array applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat, rabbit, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TrkB fusion protein Ag3790
• Full Name: neurotrophic tyrosine kinase, receptor, type 2
• Calculated Molecular Weight: 822 aa, 92 kDa
• Observed Molecular Weight: 90-95 kDa, 140-145 kDa
• GenBank Accession Number: BC031835
• Gene Symbol: TrkB
• Gene ID (NCBI): 4915
• RRID: AB_2155156
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance through regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes full length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).

Publications

Species	Application	Title
rat	WB	Chemosphere; Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny.; Authors - Mingdan You
rat	WB	Oxid Med Cell Longev; Dl-3-n-Butylphthalide Improves Neuroinflammation in Mice with Repeated Cerebral Ischemia-Reperfusion Injury through the Nrf2-Mediated Antioxidant Response and TLR4/MyD88/NF-κB Signaling Pathway.; Authors - Yaran Gao
mouse	WB	J Affect Disord; Prenatal alcohol exposure enhanced alcohol preference and susceptibility to PTSD in a sex-dependent manner through the synaptic HCN1 channel; Authors - Hui Yao
rat	WB	ACS Chem Neurosci; Tetrahydropalmatine Regulates BDNF through TrkB/CAM Interaction to Alleviate the Neurotoxicity Induced by Methamphetamine.; Authors - Lian Liu
human	IHC	Am J Cancer Res; LMP1 promotes nasopharyngeal carcinoma metastasis through NTRK2-mediated anoikis resistance.; Authors - Zhilan Li
human	WB,IF	Cell Biosci; A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation.; Authors - Juan Yin

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