Neurotrophin 3 Polyclonal antibody

Neurotrophin 3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 18084-1-AP

Synonyms

NTF3, NTF 3, NT-3, NT3, NT 3

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 18084-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 18084-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 18084-1-AP

  IP result of anti-Neurotrophin 3 (IP:18084-1-AP, 4ug; Detection:18084-1-AP 1:800) with SH-SY5Y cells lysate 1600ug.

• 

  IF Staining of SH-SY5Y using 18084-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, human placenta tissue
• Positive IP detected in: SH-SY5Y cells
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Product Information

18084-1-AP targets Neurotrophin 3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Neurotrophin 3 fusion protein Ag12798
• Full Name: neurotrophin 3
• Calculated Molecular Weight: 257 aa, 29 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC069773
• Gene Symbol: Neurotrophin 3
• Gene ID (NCBI): 4908
• RRID: AB_2298715
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IHC	ACS Appl Mater Interfaces; Surface Coating of Pulmonary siRNA Delivery Vectors Enabling Mucus Penetration, Cell Targeting, and Intracellular Radical Scavenging for Enhanced Acute Lung Injury Therapy.; Authors - Jiaojiao Zhu
human	WB	Stem Cell Res Ther; NT3P75-2 gene-modified bone mesenchymal stem cells improve neurological function recovery in mouse TBI model.; Authors - Ke Wu
human	IHC	Invest Ophthalmol Vis Sci; Alteration of Neurotrophic Factors and Innervation in Extraocular Muscles of Individuals With Concomitant Esotropia; Authors - Jie Hao
	WB	Front Pharmacol; Synergic Neuroprotection Between Ligusticum Chuanxiong Hort and Borneol Against Ischemic Stroke by Neurogenesis via Modulating Reactive Astrogliosis and Maintaining the Blood-Brain Barrier.; Authors - Bin Yu
mouse	WB	Brain Res Bull; PPARα contributes to the therapeutic effect of hydrogen gas against sepsis-associated encephalopathy with the regulation to the CREB-BDNF signaling pathway and hippocampal neuron plasticity-related gene expression.; Authors - Yuanyuan Bai
rat	WB,IHC	Neurochem Res; Human Umbilical Cord Mesenchymal Stem Cells Overexpressing Nerve Growth Factor Ameliorate Diabetic Cystopathy in Rats.; Authors - Wu WenBo